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Redundant role of DEAD box proteins p68 (Ddx5) and p72/p82 (Ddx17) in ribosome biogenesis and cell proliferation.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2007; Vol. 35 (11), pp. 3590-601. Date of Electronic Publication: 2007 May 07. - Publication Year :
- 2007
-
Abstract
- The DEAD box proteins encoded by the genes ddx5 (p68) and ddx17 (isoforms p72 and p82) are more closely related to each other than to any other member of their family. We found that p68 negatively controls p72/p82 gene expression but not vice versa. Knocking down of either gene does not affect cell proliferation, in case of p68 suppression, however, only on condition that p72/p82 overexpression was granted. In contrast, co-silencing of both genes causes perturbation of nucleolar structure and cell death. In mutant studies, the apparently redundant role(s) of p68 and p72/p82 correspond to their ability to catalyze RNA rearrangement rather than RNA unwinding reactions. In search for possible physiological targets of this RNA rearrangement activity it is shown that the nucleolytic cleavage of 32S pre-rRNA is reduced after p68 subfamily knock-down, most probably due to a failure in the structural rearrangement process within the pre-60S ribosomal subunit preceding the processing of 32S pre-rRNA.
- Subjects :
- Adenosine Triphosphate metabolism
DEAD-box RNA Helicases antagonists & inhibitors
DEAD-box RNA Helicases genetics
Gene Expression Regulation
HeLa Cells
Humans
Mutation
Phenotype
Protein Isoforms antagonists & inhibitors
Protein Isoforms genetics
Protein Isoforms physiology
RNA Interference
RNA Precursors chemistry
RNA Precursors metabolism
RNA Splicing
RNA, Ribosomal chemistry
RNA, Small Nuclear metabolism
Cell Proliferation
DEAD-box RNA Helicases physiology
RNA Processing, Post-Transcriptional
RNA, Ribosomal metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 35
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 17485482
- Full Text :
- https://doi.org/10.1093/nar/gkm058