Back to Search Start Over

High-speed mapping of synaptic connectivity using photostimulation in Channelrhodopsin-2 transgenic mice.

Authors :
Wang H
Peca J
Matsuzaki M
Matsuzaki K
Noguchi J
Qiu L
Wang D
Zhang F
Boyden E
Deisseroth K
Kasai H
Hall WC
Feng G
Augustine GJ
Source :
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2007 May 08; Vol. 104 (19), pp. 8143-8. Date of Electronic Publication: 2007 May 01.
Publication Year :
2007

Abstract

To permit rapid optical control of brain activity, we have engineered multiple lines of transgenic mice that express the light-activated cation channel Channelrhodopsin-2 (ChR2) in subsets of neurons. Illumination of ChR2-positive neurons in brain slices produced photocurrents that generated action potentials within milliseconds and with precisely timed latencies. The number of light-evoked action potentials could be controlled by varying either the amplitude or duration of illumination. Furthermore, the frequency of light-evoked action potentials could be precisely controlled up to 30 Hz. Photostimulation also could evoke synaptic transmission between neurons, and, by scanning with a small laser light spot, we were able to map the spatial distribution of synaptic circuits connecting neurons within living cerebral cortex. We conclude that ChR2 is a genetically based photostimulation technology that permits analysis of neural circuits with high spatial and temporal resolution in transgenic mammals.

Details

Language :
English
ISSN :
0027-8424
Volume :
104
Issue :
19
Database :
MEDLINE
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Publication Type :
Academic Journal
Accession number :
17483470
Full Text :
https://doi.org/10.1073/pnas.0700384104