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Mass spectrometry-based strategy for direct detection and quantification of some mycotoxins produced by Stachybotrys and Aspergillus spp. in indoor environments.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2007 Jul; Vol. 73 (13), pp. 4211-7. Date of Electronic Publication: 2007 May 04. - Publication Year :
- 2007
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Abstract
- Dampness in buildings has been linked to adverse health effects, but the specific causative agents are unknown. Mycotoxins are secondary metabolites produced by molds and toxic to higher vertebrates. In this study, mass spectrometry was used to demonstrate the presence of mycotoxins predominantly produced by Aspergillus spp. and Stachybotrys spp. in buildings with either ongoing dampness or a history of water damage. Verrucarol and trichodermol, hydrolysis products of macrocyclic trichothecenes (including satratoxins), and trichodermin, predominately produced by Stachybotrys chartarum, were analyzed by gas chromatography-tandem mass spectrometry, whereas sterigmatocystin (mainly produced by Aspergillus versicolor), satratoxin G, and satratoxin H were analyzed by high-performance liquid chromatography-tandem mass spectrometry. These mycotoxin analytes were demonstrated in 45 of 62 building material samples studied, in three of eight settled dust samples, and in five of eight cultures of airborne dust samples. This is the first report on the use of tandem mass spectrometry for demonstrating mycotoxins in dust settled on surfaces above floor level in damp buildings. The direct detection of the highly toxic sterigmatocystin and macrocyclic trichothecene mycotoxins in indoor environments is important due to their potential health impacts.
- Subjects :
- Air Pollution, Indoor analysis
Aspergillus pathogenicity
Chromatography, High Pressure Liquid standards
Construction Materials analysis
Dust analysis
Gas Chromatography-Mass Spectrometry standards
Humans
Mycotoxins standards
Mycotoxins toxicity
Reference Standards
Spectrometry, Mass, Electrospray Ionization standards
Stachybotrys pathogenicity
Sterigmatocystin analysis
Tandem Mass Spectrometry standards
Trichodermin analysis
Trichothecenes analysis
Aspergillus chemistry
Mycotoxins analysis
Stachybotrys chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 73
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 17483261
- Full Text :
- https://doi.org/10.1128/AEM.00343-07