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Deletion and substitution analysis of the Escherichia coli TonB Q160 region.
- Source :
-
Journal of bacteriology [J Bacteriol] 2007 Jul; Vol. 189 (13), pp. 4662-70. Date of Electronic Publication: 2007 May 04. - Publication Year :
- 2007
-
Abstract
- The active transport of iron siderophores and vitamin B(12) across the outer membrane (OM) of Escherichia coli requires OM transporters and the potential energy of the cytoplasmic membrane (CM) proton gradient and CM proteins TonB, ExbB, and ExbD. A region at the amino terminus of the transporter, called the TonB box, directly interacts with TonB Q160 region residues. R158 and R166 in the TonB Q160 region were proposed to play important roles in cocrystal structures of the TonB carboxy terminus with OM transporters BtuB and FhuA. In contrast to predictions based on the crystal structures, none of the single, double, or triple alanyl substitutions at arginyl residues significantly decreased TonB activity. Even the quadruple R154A R158A R166A R171A mutant TonB still retained 30% of wild-type activity. Up to five residues centered on TonB Q160 could be deleted without inactivating TonB or preventing its association with the OM. TonB mutant proteins with nested deletions of 7, 9, or 11 residues centered on TonB Q160 were inactive and appeared never to have associated with the OM. Because the 7-residue-deletion mutant protein (TonBDelta7, lacking residues S157 to Y163) could still form disulfide-linked dimers when combined with W213C or F202C in the TonB carboxy terminus, the TonBDelta7 deletion did not prevent necessary energy-dependent conformational changes that occur in the CM. Thus, it appeared that initial contact with the OM is made through TonB residues S157 to Y163. It is hypothesized that the TonB Q160 region may be part of a large disordered region required to span the periplasm and contact an OM transporter.
- Subjects :
- Alanine genetics
Alanine metabolism
Amino Acid Sequence
Amino Acid Substitution
Arginine genetics
Arginine metabolism
Bacterial Outer Membrane Proteins genetics
Bacterial Outer Membrane Proteins metabolism
Bacteriophages growth & development
Carrier Proteins genetics
Carrier Proteins metabolism
Cell Membrane metabolism
Colicins pharmacology
Electrophoresis, Polyacrylamide Gel
Escherichia coli drug effects
Escherichia coli virology
Escherichia coli Proteins metabolism
Gene Deletion
Immunoblotting
Membrane Proteins metabolism
Molecular Sequence Data
Protein Binding
Receptors, Cell Surface genetics
Receptors, Cell Surface metabolism
Escherichia coli genetics
Escherichia coli Proteins genetics
Membrane Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 189
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 17483231
- Full Text :
- https://doi.org/10.1128/JB.00180-07