Back to Search
Start Over
Inactivation of viruses on surfaces by ultraviolet germicidal irradiation.
- Source :
-
Journal of occupational and environmental hygiene [J Occup Environ Hyg] 2007 Jun; Vol. 4 (6), pp. 400-5. - Publication Year :
- 2007
-
Abstract
- In many outbreaks caused by viruses, the transmission of the agents can occur through contaminated environmental surfaces. Because of the increasing incidence of viral infections, there is a need to evaluate novel engineering control methods for inactivation of viruses on surfaces. Ultraviolet germicidal irradiation (UVGI) is considered a promising method to inactivate viruses. This study evaluated UVGI effectiveness for viruses on the surface of gelatin-based medium in a UV exposure chamber. The effects of UV dose, viral nucleic acid type (single-stranded RNA, ssRNA; single-stranded DNA, ssDNA; double-stranded RNA, dsRNA; and double-stranded DNA, dsDNA), and relative humidity on the virus survival fraction were investigated. For 90% viral reduction, the UV dose was 1.32 to 3.20 mJ/cm2 for ssRNA, 2.50 to to 4.47 mJ/cm2 for ssDNA, 3.80 to 5.36 mJ/cm2 for dsRNA, and 7.70 to 8.13 mJ/cm2 for dsDNA. For all four tested viruses, the UV dose for 99% viral reduction was 2 times higher than those for 90% viral reduction. Viruses on a surface with single-stranded nucleic acid (ssRNA and ssDNA) were more susceptible to UV inactivation than viruses with double-stranded nucleic acid (dsRNA and dsDNA). For the same viral reduction, the UV dose at 85% relative humidity (RH) was higher than that at 55% RH. In summary, results showed that UVGI was an effective method for inactivation of viruses on surfaces.
- Subjects :
- Bacteriophage T7 physiology
Bacteriophage phi 6 physiology
Bacteriophage phi X 174 physiology
DNA, Single-Stranded radiation effects
DNA, Viral radiation effects
Escherichia coli virology
Humidity
Levivirus physiology
Pseudomonas syringae virology
RNA, Double-Stranded radiation effects
RNA, Viral radiation effects
Virus Inactivation radiation effects
Bacteriophage T7 radiation effects
Bacteriophage phi 6 radiation effects
Bacteriophage phi X 174 radiation effects
Levivirus radiation effects
Ultraviolet Rays
Subjects
Details
- Language :
- English
- ISSN :
- 1545-9624
- Volume :
- 4
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of occupational and environmental hygiene
- Publication Type :
- Academic Journal
- Accession number :
- 17474029
- Full Text :
- https://doi.org/10.1080/15459620701329012