Back to Search
Start Over
Thioredoxin from the silkworm, Bombyx mori: cDNA sequence, expression, and functional characterization.
- Source :
-
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2007 Jul; Vol. 147 (3), pp. 574-81. Date of Electronic Publication: 2007 Mar 31. - Publication Year :
- 2007
-
Abstract
- A thioredoxin (Trx) gene was cloned from the silkworm, Bombyx mori. The B. mori Trx (BmTrx) cDNA contains an open reading frame of 318 bp encoding 106 amino acid residues with a conserved active site (CGPC). Northern blot analysis revealed the presence of BmTrx transcripts in all tissues examined. The cDNA encoding BmTrx was expressed as a 12-kDa polypeptide in baculovirus-infected insect Sf9 cells. The recombinant BmTrx proved to be biologically active, using an insulin reduction assay, and was also able to activate thioredoxin peroxidase from B. mori. When H2O2 or paraquat was injected into the body cavity of B. mori larvae, BmTrx mRNA expression was upregulated in the fat body tissue. In addition, the expression levels of BmTrx mRNA in the fat body were greatly increased when B. mori larvae were exposed to low or high temperatures, or injected with microorganisms. These results suggest that BmTrx possibly protects against oxidative stress caused by extreme temperatures and microbial infection as well as by intracellularly generated reactive oxygen species during metabolism.
- Subjects :
- Animals
Bombyx metabolism
DNA, Complementary genetics
Enzyme Activation drug effects
Enzyme Activation physiology
Gene Expression Regulation drug effects
Herbicides pharmacology
Hydrogen Peroxide pharmacology
Insect Proteins biosynthesis
Organ Specificity drug effects
Organ Specificity physiology
Oxidants pharmacology
Oxidative Stress drug effects
Oxidative Stress physiology
Paraquat pharmacology
Peroxidases metabolism
Peroxiredoxins
Reactive Oxygen Species metabolism
Thioredoxins biosynthesis
Bombyx genetics
Gene Expression Regulation physiology
Insect Proteins genetics
Thioredoxins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1096-4959
- Volume :
- 147
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 17466556
- Full Text :
- https://doi.org/10.1016/j.cbpb.2007.03.012