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Gamma-irradiated ceruloplasmin affords antifibrillatory protection against ischemia/reperfusion damage in the isolated rat heart.
- Source :
-
International journal of radiation biology [Int J Radiat Biol] 2007 May; Vol. 83 (5), pp. 309-17. - Publication Year :
- 2007
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Abstract
- Purpose: Ceruloplasmin (CP), an important serum antioxidant, was previously found to reduce the incidence of ventricular fibrillation (VF) induced by ischemia and reperfusion in isolated rat hearts. The present study investigated whether CP sterilized by gamma-irradiation maintains its antiarrhythmic capacity and in vitro antioxidant properties.<br />Materials and Methods: Isolated rat hearts submitted to regional ischemia (15 min), were reperfused (10 min) with native CP or with CP irradiated at various doses (1-3 kGy) in the absence or presence of tyrosine (Tyr).<br />Results: All untreated hearts showed VF at reperfusion, which were all irreversible ventricular fibrillation (IVF). No IVF were found in hearts treated with native CP or gamma-irradiated CP. Cardioprotection afforded by irradiated CP (with or without Tyr) was slightly higher than that obtained with native CP. No VF at all (100% prevention) was found in hearts treated with CP irradiated alone or in the presence of tyrosine at 3 kGy. Tyrosine and irradiated tyrosine had no cardiotoxic or protective effects on reperfusion-induced arrhythmias. The Oxygen Radical Absorbing Capacity (ORAC), measured in vitro with beta-phycoerythrin (beta-PE) fluorescent indicator, was slightly higher for gamma-irradiated CP in the presence of Tyr.<br />Conclusions: Ceruloplasmin sterilized by gamma-irradiation maintains antioxidant and antiarrhythmic effects in the post-ischemia reperfused isolated rat heart.
- Subjects :
- Animals
Anti-Arrhythmia Agents pharmacology
Anti-Arrhythmia Agents radiation effects
Antioxidants pharmacology
Antioxidants radiation effects
Dose-Response Relationship, Radiation
Heart physiopathology
Heart Rate drug effects
In Vitro Techniques
Myocardial Reperfusion Injury complications
Myocardial Reperfusion Injury physiopathology
Rats
Tyrosine pharmacology
Tyrosine radiation effects
Ventricular Fibrillation etiology
Ceruloplasmin pharmacology
Ceruloplasmin radiation effects
Gamma Rays
Heart drug effects
Myocardial Reperfusion Injury prevention & control
Ventricular Fibrillation prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 0955-3002
- Volume :
- 83
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International journal of radiation biology
- Publication Type :
- Academic Journal
- Accession number :
- 17457756
- Full Text :
- https://doi.org/10.1080/09553000701311229