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Use of a genetically defined double mutant strain of Bordetella bronchiseptica lacking adenylate cyclase and type III secretion as a live vaccine.
- Source :
-
Infection and immunity [Infect Immun] 2007 Jul; Vol. 75 (7), pp. 3665-72. Date of Electronic Publication: 2007 Apr 23. - Publication Year :
- 2007
-
Abstract
- While most vaccines consisting of killed bacteria induce high serum antibody titers, they do not always confer protection as effective as that induced by infection, particularly against mucosal pathogens. Bordetella bronchiseptica is a gram-negative respiratory pathogen that is endemic in many nonhuman mammalian populations and causes substantial disease in a variety of animals. At least 14 different live attenuated vaccines against this pathogen are available for use in a variety of livestock and companion animals. However, there are few published data on the makeup or efficacy of these vaccines. Here we report the use of a genetically engineered double mutant of B. bronchiseptica, which lacks adenylate cyclase and type III secretion, as a vaccine candidate. This strain is safe at high doses, even for highly immunocompromised animals, and induces immune responses that are protective against highly divergent B. bronchiseptica strains, preventing colonization in the lower respiratory tract and decreasing the bacterial burden in the upper respiratory tract. This novel B. bronchiseptica vaccine candidate induces strong local immunity while eliminating damage caused by the two predominant cytotoxic mechanisms.
- Subjects :
- Adenylyl Cyclases genetics
Adenylyl Cyclases metabolism
Animals
Bacterial Proteins genetics
Bacterial Proteins metabolism
Bordetella Infections immunology
Bordetella Infections microbiology
Bordetella bronchiseptica enzymology
Bordetella bronchiseptica genetics
Bordetella parapertussis immunology
Bordetella pertussis immunology
Genetic Engineering methods
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Respiratory System microbiology
Whooping Cough immunology
Whooping Cough prevention & control
Adenylyl Cyclases immunology
Antibodies, Bacterial blood
Bacterial Vaccines administration & dosage
Bacterial Vaccines genetics
Bacterial Vaccines immunology
Bordetella Infections prevention & control
Bordetella bronchiseptica immunology
Mutation
Vaccines, Attenuated administration & dosage
Vaccines, Attenuated genetics
Vaccines, Attenuated immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 75
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 17452472
- Full Text :
- https://doi.org/10.1128/IAI.01648-06