Back to Search
Start Over
The effect of sevoflurane on the release of [3H]dopamine from rat brain cortical slices.
- Source :
-
Brain research bulletin [Brain Res Bull] 2007 May 30; Vol. 72 (4-6), pp. 309-14. Date of Electronic Publication: 2007 Feb 12. - Publication Year :
- 2007
-
Abstract
- Dopamine is a neurotransmitter that exerts major control on important brain functions and some lines of studies suggest that dopaminergic neurotransmission may be a potential target for volatile anesthetics. In the present study, rat brain cortical slices were labeled with [(3)H]dopamine to investigate the effects of sevoflurane on the release of this neurotransmitter. [(3)H]dopamine release was significantly increased in the presence of sevoflurane (0.46 mM) and this effect was independent of extracellular or intracellular calcium. In addition, [(3)H]dopamine release evoked by sevoflurane was not affected by TTX (blocker of voltage-dependent sodium channels) or reserpine (a blocker of the vesicular monoamine transporter). These data suggest that the dopamine release induced by sevoflurane is non-vesicular, independent of exocytosis and, would be mediated by the dopamine transporter (DAT). GBR12909 and nomifensine, inhibitors of DAT, decreased the release of [(3)H]dopamine evoked by sevoflurane. The same effect was also observed when the brain cortical slices were incubated at low temperature and low extracellular sodium. Ouabain, a Na(+)/K(+) ATPase pump inhibitor, which is known to induce dopamine release through reverse transport, decreased [(3)H]dopamine release induced by sevoflurane. In conclusion, the present study suggests that sevoflurane increases [(3)H]dopamine release in brain cortical slices that is mediated by DAT located at the plasma membrane.
- Subjects :
- Adrenergic Uptake Inhibitors pharmacology
Animals
Chelating Agents pharmacology
Dose-Response Relationship, Drug
Drug Interactions
Enzyme Inhibitors pharmacology
Female
In Vitro Techniques
Male
Rats
Rats, Wistar
Reserpine pharmacology
Sevoflurane
Sodium pharmacology
Tritium metabolism
Cerebral Cortex drug effects
Dopamine metabolism
Methyl Ethers pharmacology
Platelet Aggregation Inhibitors pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0361-9230
- Volume :
- 72
- Issue :
- 4-6
- Database :
- MEDLINE
- Journal :
- Brain research bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 17452291
- Full Text :
- https://doi.org/10.1016/j.brainresbull.2007.01.011