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Tendon and bone responses to a collagen-coated suture material.
- Source :
-
Journal of shoulder and elbow surgery [J Shoulder Elbow Surg] 2007 Sep-Oct; Vol. 16 (5 Suppl), pp. S222-30. Date of Electronic Publication: 2007 Apr 19. - Publication Year :
- 2007
-
Abstract
- Tendon to bone integration after rotator cuff repair is not a reproducible process. During repair, bioabsorbable and nonabsorbable suture material is universally used to facilitate the procedure. Improving the biological architecture of inert suture might aid in overall tendon to bone healing. The objective of our study is to enhance the bone to tendon union by absorbing type I collagen onto high strength nonabsorbable polyester/polyethylene suture commonly used in rotator cuff surgery. Our purpose was to evaluate the tendon and bone cellular response to this novel coated suture compared to uncoated suture. Primary human osteoblasts (HOBs) and tenocytes were plated onto polyester/polyethylene suture that was either uncoated or coated with type I bovine collagen. Cell adhesion to the sutures was assayed at 24 hours. Proliferation was determined at 48 hours by measuring [3H]- Thymidine incorporation in cells attached to the sutures. At 24 and 48 hours, respectively, cells grown on the collagen-coated suture showed a significantly greater response measured by adhesion and proliferation than cells grown on uncoated suture. At five days of culture, alkaline phosphatase activity and protein synthesis was significantly greater on the collagen-coated suture compared to uncoated. Collagen-coated polyester/polyethylene suture appears to stimulate adhesion, proliferation alkaline phosphatase, and protein synthesis more than uncoated sutures, and therefore may aid in the tendon to bone incorporation process critical to rotator cuff repair.
- Subjects :
- Adolescent
Adult
Alkaline Phosphatase analysis
Biomechanical Phenomena
Bone and Bones cytology
Cell Adhesion drug effects
Cell Differentiation drug effects
Cell Proliferation drug effects
Cells, Cultured
Coated Materials, Biocompatible
Collagen Type I
Humans
Male
Microscopy, Electron, Scanning
Middle Aged
Osteoblasts drug effects
Rotator Cuff
Sutures
Tendons cytology
Bone and Bones drug effects
Tendons drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1532-6500
- Volume :
- 16
- Issue :
- 5 Suppl
- Database :
- MEDLINE
- Journal :
- Journal of shoulder and elbow surgery
- Publication Type :
- Academic Journal
- Accession number :
- 17448699
- Full Text :
- https://doi.org/10.1016/j.jse.2007.02.113