Back to Search
Start Over
Probing the cavity of the slow inactivated conformation of shaker potassium channels.
- Source :
-
The Journal of general physiology [J Gen Physiol] 2007 May; Vol. 129 (5), pp. 403-18. Date of Electronic Publication: 2007 Apr 16. - Publication Year :
- 2007
-
Abstract
- Slow inactivation involves a local rearrangement of the outer mouth of voltage-gated potassium channels, but nothing is known regarding rearrangements in the cavity between the activation gate and the selectivity filter. We now report that the cavity undergoes a conformational change in the slow-inactivated state. This change is manifest as altered accessibility of residues facing the aqueous cavity and as a marked decrease in the affinity of tetraethylammonium for its internal binding site. These findings have implications for global alterations of the channel during slow inactivation and putative coupling between activation and slow-inactivation gates.
- Subjects :
- Binding Sites
Cadmium metabolism
Cell Line
Humans
Kinetics
Membrane Potentials
Models, Biological
Models, Molecular
Mutagenesis, Site-Directed
Patch-Clamp Techniques
Potassium Channel Blockers metabolism
Protein Conformation
Shaker Superfamily of Potassium Channels chemistry
Shaker Superfamily of Potassium Channels genetics
Tetraethylammonium metabolism
Transfection
Ion Channel Gating
Potassium metabolism
Shaker Superfamily of Potassium Channels metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1295
- Volume :
- 129
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of general physiology
- Publication Type :
- Academic Journal
- Accession number :
- 17438120
- Full Text :
- https://doi.org/10.1085/jgp.200709758