A sensitive sandwich ELISA for buffalo prolactin.

Two murine monoclonal antibodies have been developed against the buffalo prolactin (buPRL). These were designated as 1501 and 1504. Using two MAbs and anti-buPRL rabbit serum, an analysis was performed for the development of a sandwich ELISA (sELISA). The 1504/buPRL/anti-buPRL rabbit serum system was found feasible for sELISA. The sELISA had a sensitivity of 156 pg/mL. The buffalo serum sample showed a parallelism with the standard curve. The intra- and inter-assay coefficients of variance were 8.4% and 9.06%, respectively. These data proved the validity of the assay.