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Insulin and amino acid availability regulate atrogin-1 in avian QT6 cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2007 May 25; Vol. 357 (1), pp. 181-6. Date of Electronic Publication: 2007 Mar 30. - Publication Year :
- 2007
-
Abstract
- New evidence has demonstrated that the expression of major genes, termed atrogenes, controls the ubiquitin-proteasome proteolytic pathway. The present work aimed to study the impact of insulin and amino acids on the expression of one of these atrogenes, the E3 ubiquitin ligase Muscle Atrophy F box (MAFbx, also called atrogin-1), in quail muscle (QT6) fibroblasts. First, we characterized atrogin-1 in QT6 cells and demonstrated the insulin sensitivity of these cells. Second, we showed that insulin reduced atrogin-1 mRNA via the phosphatidylinositol-3'kinase (PI3K)/protein kinase B (PKB or AKT)/target of rapamycin (TOR) pathway. Atrogin-1 expression also depended on the availability of an individual amino acid, i.e., methionine. Moreover, the amino acid-induced reduction of atrogin-1 was inhibited by rapamycin, indicating the involvement of the TOR pathway in such regulation. In conclusion, expression of the ubiquitin ligase atrogin-1 is regulated by both insulin and amino acids through the TOR pathway.
- Subjects :
- Animals
Cell Line
Fibroblasts drug effects
Gene Expression Regulation drug effects
Gene Expression Regulation physiology
Quail
Signal Transduction drug effects
Amino Acids administration & dosage
Fibroblasts metabolism
Insulin administration & dosage
Muscle Proteins metabolism
SKP Cullin F-Box Protein Ligases metabolism
Signal Transduction physiology
Ubiquitin-Protein Ligases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 357
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 17418104
- Full Text :
- https://doi.org/10.1016/j.bbrc.2007.03.131