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Development of a sensitive liquid chromatography method coupled with a tandem mass spectrometric detection for the clinical analysis of vinflunine and 4-O-deacetyl vinflunine in blood, urine and faeces.
- Source :
-
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2007 Jun 15; Vol. 853 (1-2), pp. 294-302. Date of Electronic Publication: 2007 Mar 31. - Publication Year :
- 2007
-
Abstract
- A sensitive and specific liquid chromatographic method coupled with tandem mass spectrometric detection was set up and fully validated for the simultaneous quantification of vinflunine (VFL) and its pharmacologically active metabolite, 4-O-deacetyl vinflunine (DVFL). The two compounds, as well as vinblastine (used as internal standard), were deproteinised from blood and faeces, analysed on a cyano type column and detected on a Micromass Quattro II system in the positive ion mode after ionisation using an electrospray ion source. In blood, linearity was assessed up to 200 ng/ml for vinflunine and 100 ng/ml for 4-O-deacetyl vinflunine. The lower limit of quantification was validated at 250 pg/ml for both compounds. In other biological media, the linearity was assessed within the same range; the limit of quantification was adjusted according to the expected concentration levels of each compound. This method was first developed in order to identify the structures and to elucidate the metabolic pathway of vinflunine. Thanks to its high sensitivity and specificity, the method has enabled the quantification of vinflunine and 4-O-deacetyl vinflunine in blood at trace levels, and has contributed to the knowledge of vinflunine metabolism by monitoring up to 10 metabolites.
- Subjects :
- Humans
Molecular Structure
Reproducibility of Results
Vinblastine analogs & derivatives
Vinblastine blood
Vinblastine chemistry
Vinblastine urine
Chromatography, High Pressure Liquid methods
Feces chemistry
Spectrometry, Mass, Electrospray Ionization methods
Tandem Mass Spectrometry methods
Vinblastine analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1570-0232
- Volume :
- 853
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 17416213
- Full Text :
- https://doi.org/10.1016/j.jchromb.2007.03.030