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Transduction with human telomerase reverse transcriptase immortalizes a rhesus macaque CD8+ T cell clone with maintenance of surface marker phenotype and function.
- Source :
-
AIDS research and human retroviruses [AIDS Res Hum Retroviruses] 2007 Mar; Vol. 23 (3), pp. 456-65. - Publication Year :
- 2007
-
Abstract
- T cell lines and clones play a key role in basic studies of cellular immunology, and are also finding applications in adoptive immunotherapy. However, with proliferative expansion, T cells ultimately undergo cellular senescence and death, so that long-term culture of T cell clones is difficult to achieve. Expression of telomerase reverse transcriptase (TERT) in differentiated cells can maintain telomere length over many cell divisions, preventing senescence. We used a retroviral vector that expresses the human TERT (hTERT) gene to transduce a rhesus macaque-derived CD8(+) T cell clone specific for the MamuA*01-restricted immunodominant SIV gag epitope CM9. Extensive in vitro characterization revealed that the untransduced parental cells and the hTERT-transduced cells displayed comparable proliferation capacity, effector memory surface marker profiles, cytolytic activities, and cytokine profiles following antigen stimulation. The hTERT-transduced cells showed improved survival compared to parallel nontransduced cultures during in vitro propagation in long-term culture. Such immortalized T cells may be useful as a source of consistent controls for in vitro assays of cellular immune function, and as a potentially important reagent for autologous adoptive cellular immunotherapy studies in macaques.
- Subjects :
- Animals
Cell Line
Clone Cells immunology
Clone Cells metabolism
Female
Humans
T-Lymphocytes, Cytotoxic virology
CD8-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes metabolism
CD8-Positive T-Lymphocytes virology
Macaca mulatta virology
Simian Immunodeficiency Virus immunology
T-Lymphocytes, Cytotoxic metabolism
Telomerase metabolism
Transduction, Genetic methods
Subjects
Details
- Language :
- English
- ISSN :
- 0889-2229
- Volume :
- 23
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- AIDS research and human retroviruses
- Publication Type :
- Academic Journal
- Accession number :
- 17411379
- Full Text :
- https://doi.org/10.1089/aid.2006.0194