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Oligonucleotide array analysis of cyclic tension-responsive genes in human periodontal ligament fibroblasts.
- Source :
-
The international journal of biochemistry & cell biology [Int J Biochem Cell Biol] 2007; Vol. 39 (5), pp. 910-21. Date of Electronic Publication: 2007 Jan 23. - Publication Year :
- 2007
-
Abstract
- Mechanical stress results in differential gene expression that is critical to convert the stimulus into biochemical signals. Under physiological stress such as occlusal force, human periodontal ligament fibroblasts (HPLF) are associated with homeostasis of periodontal tissues however the changes in response to mechanotransduction remain uncharacterized. We hypothesized that cyclic tension-responsive (CT) genes may be used to identify a set of fundamental pathways of mechanotransduction. Our goal was to catalogue CT genes in cultured HPLF. HPLF were subjected to cyclic tension up to 16h, and total RNA was isolated from both tension-loaded and static HPLF. The oligonucleotide arrays analysis revealed significant changes of mRNA accumulation for 122 CT genes, and their kinetics were assigned by the K-means clustering methods. Ingenuity Pathway Analysis was completed for HPLF mechanotransduction using 50 CT genes. This analysis revealed that cyclic tension immediately down-regulated all nuclear transcription factors except v-fos FBJ murine osteosarcoma viral oncogene homolog (FOS) reacting as an early responsive gene. In turn, transcription factors such as tumor protein p53 binding protein 2 (TP53BP2), and extra-nuclear molecules such as adrenergic receptor beta2 (ADRB2) were up-regulated after 1-2h, which may result in fundamental HPLF functions to adapt to cyclic tension. Subsequent inhibition assays using Y27632, a pharmacologic inhibitor of Rho-associated kinase (ROCK), suggested that HPLF has both ROCK-dependent and ROCK-independent CT genes. Mechanical stress was found to effect the expression of numerous genes, in particular, expression of an early responsive gene; FOS initiates alteration of HPLF behaviors to control homeostasis of the periodontal ligament.
- Subjects :
- Adolescent
Adult
Amides pharmacology
Bite Force
Cells, Cultured
Enzyme Inhibitors pharmacology
Female
Fibroblasts cytology
Gene Expression Regulation drug effects
Humans
Intracellular Signaling Peptides and Proteins antagonists & inhibitors
Intracellular Signaling Peptides and Proteins genetics
Intracellular Signaling Peptides and Proteins metabolism
Male
Models, Biological
Periodontal Ligament cytology
Protein Serine-Threonine Kinases antagonists & inhibitors
Protein Serine-Threonine Kinases genetics
Protein Serine-Threonine Kinases metabolism
Pyridines pharmacology
Stress, Mechanical
rho-Associated Kinases
Fibroblasts metabolism
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis methods
Periodontal Ligament metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1357-2725
- Volume :
- 39
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The international journal of biochemistry & cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 17409011
- Full Text :
- https://doi.org/10.1016/j.biocel.2007.01.015