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[Role of c-Jun NH (2)-terminal kinase in insulin resistance after burn].
- Source :
-
Zhonghua wai ke za zhi [Chinese journal of surgery] [Zhonghua Wai Ke Za Zhi] 2007 Jan 01; Vol. 45 (1), pp. 62-4. - Publication Year :
- 2007
-
Abstract
- Objective: To investigate the role of c-Jun NH (2)-terminal kinase (JNk) in insulin resistance after burn and its mechanism.<br />Methods: Twenty-four Sprague-Dawley rats were randomized to control, burn and burn + anisomycin groups. The rats in control group received sham burn trauma, and burn and burn + anisomycin groups received 30% total body surface area (TBSA) full thickness burn injury. Anisomycin (5 mg/kg) together with 250 microl dimethyl sulfoxide (DMSO) was injected to the rats in anisomycin group intravenously, and only 250 microl DMSO in the other two groups. Euglycemic-hyperinsulinemic glucose clamps was performed 2 hours after the injection. The changes of phospho-serine 307, phospho-tyrosine of insulin receptor substrate (IRS)-1 and phospho-JNK in muscle tissues were determined and compared using immunoprecipitation and Western blot analysis or immunohistochemistry in the three groups.<br />Results: The infusing rates of total 10% glucose (mg x kg(-1) x min(-1)) in control, burn and burn + anisomycin group were 12.3 +/- 0.4, 6.6 +/- 0.3, 6.5 +/- 0.4, respectively. The level of IRS-1 Serine 307 phosphorylation and phospho-JNK in muscle increased significantly, while insulin-induced tyrosine phosphorylation of IRS-1 decreased markedly after burn.<br />Conclusions: The activation of JNK elevates the level of IRS-1 phospho-serine 307 and might play a role in insulin resistance after burn in rats.
- Subjects :
- Adaptor Proteins, Signal Transducing metabolism
Animals
Anisomycin administration & dosage
Anti-Bacterial Agents administration & dosage
Blotting, Western
Burns enzymology
Burns metabolism
Dimethyl Sulfoxide administration & dosage
Disease Models, Animal
Female
Glucose Clamp Technique
Immunohistochemistry
Injections, Intravenous
Insulin administration & dosage
Insulin Receptor Substrate Proteins
Male
Muscles metabolism
Phosphorylation drug effects
Random Allocation
Rats
Rats, Sprague-Dawley
Serine metabolism
Tyrosine metabolism
Burns physiopathology
Insulin Resistance physiology
JNK Mitogen-Activated Protein Kinases metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0529-5815
- Volume :
- 45
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Zhonghua wai ke za zhi [Chinese journal of surgery]
- Publication Type :
- Academic Journal
- Accession number :
- 17403295