Back to Search
Start Over
Error-free RAD52 pathway and error-prone REV3 pathway determines spontaneous mutagenesis in Saccharomyces cerevisiae.
- Source :
-
Genes & genetic systems [Genes Genet Syst] 2007 Feb; Vol. 82 (1), pp. 35-42. - Publication Year :
- 2007
-
Abstract
- Using the CAN1 gene in haploid cells or heterozygous diploid cells, we characterized the effects of mutations in the RAD52 and REV3 genes of Saccharomyces cerevisiae in spontaneous mutagenesis. The mutation rate was 5-fold higher in the haploid rad52 strain and 2.5-fold lower in rev3 than in the wild-type strain. The rate in the rad52 rev3 strain was as low as in the wild-type strain, indicating the rad52 mutator phenotype to be dependent on REV3. Sequencing indicated that G:C-->T:A and G:C-->C:G transversions increased in the rad52 strain and decreased in the rev3 and rad52 rev3 strains, suggesting a role for REV3 in transversion mutagenesis. In diploid rev3 cells, frequencies of can1Delta::LEU2/can1Delta::LEU2 from CAN1/can1Delta::LEU2 due to recombination were increased over the wild-type level. Overall, in the absence of RAD52, REV3-dependent base-substitutions increased, while in the absence of REV3, RAD52-dependent recombination events increased. We further found that the rad52 mutant had an increased rate of chromosome loss and the rad52 rev3 double mutant had an enhanced chromosome loss mutator phenotype. Taken together, our study indicates that the error-free RAD52 pathway and error-prone REV3 pathway for rescuing replication fork arrest determine spontaneous mutagenesis, recombination, and genome instability.
- Subjects :
- Amino Acid Transport Systems, Basic genetics
Amino Acid Transport Systems, Basic metabolism
DNA Mutational Analysis
DNA-Directed DNA Polymerase metabolism
Diploidy
Haploidy
Loss of Heterozygosity
Phenotype
Rad52 DNA Repair and Recombination Protein metabolism
Recombination, Genetic
Saccharomyces cerevisiae enzymology
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins metabolism
Signal Transduction genetics
DNA-Directed DNA Polymerase genetics
Mutagenesis physiology
Mutation
Rad52 DNA Repair and Recombination Protein genetics
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1341-7568
- Volume :
- 82
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genes & genetic systems
- Publication Type :
- Academic Journal
- Accession number :
- 17396018
- Full Text :
- https://doi.org/10.1266/ggs.82.35