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Morphological and contractile characteristics of rat aortae perfused for 3 or 6 days in vitro.
- Source :
-
Artery [Artery] 1992; Vol. 19 (1), pp. 14-38. - Publication Year :
- 1992
-
Abstract
- We studied the morphological and contractile characteristics of rat thoracic aortic segments perfused for 3 or 6 days under pulsatile conditions. Light microscopic examination of the segments revealed the presence of an unchanged tunica media. However, the intimal surface was mostly devoid of endothelial cells. The perfused aortic segments showed a dramatic increase in spontaneous tone when compared to fresh and sham-treated aortic segments. Maximum responses to potassium and norepinephrine were reduced after 3 days of perfusion (20-40% reduction), while maximum responses to 5-hydroxytryptamine and angiotensin II were not significantly different. After 6 days of perfusion, maximum responses to all agonist were reduced (50-60%). Sensitivity to norepinephrine was not affected by the treatment, while sensitivity to 5-hydroxytryptamine was reduced. The perfused aortic segments relaxed well in response to isoproterenol. Our system provides a useful experimental model for short-term studies of hypertension- and atherosclerosis-related vascular changes. Further refinement and characterization could improve the performance of the system for longer-term studies.
- Subjects :
- Acetylcholine pharmacology
Angiotensin II pharmacology
Animals
Aorta, Thoracic cytology
Aorta, Thoracic drug effects
In Vitro Techniques
Isoproterenol pharmacology
Kinetics
Male
Muscle, Smooth, Vascular cytology
Muscle, Smooth, Vascular drug effects
Nitroprusside pharmacology
Perfusion instrumentation
Perfusion methods
Potassium pharmacology
Rats
Rats, Inbred Strains
Serotonin pharmacology
Time Factors
Aorta, Thoracic physiology
Isometric Contraction drug effects
Muscle, Smooth, Vascular physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0098-6127
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Artery
- Publication Type :
- Academic Journal
- Accession number :
- 1739386