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Bub1 is essential for assembly of the functional inner centromere.
- Source :
-
The Journal of cell biology [J Cell Biol] 2007 Mar 26; Vol. 176 (7), pp. 919-28. - Publication Year :
- 2007
-
Abstract
- During mitosis, the inner centromeric region (ICR) recruits protein complexes that regulate sister chromatid cohesion, monitor tension, and modulate microtubule attachment. Biochemical pathways that govern formation of the inner centromere remain elusive. The kinetochore protein Bub1 was shown to promote assembly of the outer kinetochore components, such as BubR1 and CENP-F, on centromeres. Bub1 was also implicated in targeting of Shugoshin (Sgo) to the ICR. We show that Bub1 works as a master organizer of the ICR. Depletion of Bub1 from Xenopus laevis egg extract or from HeLa cells resulted in both destabilization and displacement of chromosomal passenger complex (CPC) from the ICR. Moreover, soluble Bub1 controls the binding of Sgo to chromatin, whereas the CPC restricts loading of Sgo specifically onto centromeres. We further provide evidence that Bub1 kinase activity is pivotal for recruitment of all of these components. Together, our findings demonstrate that Bub1 acts at multiple points to assure the correct kinetochore formation.
- Subjects :
- Animals
Cell Cycle Proteins metabolism
Cell Nucleus genetics
Centromere ultrastructure
Chromosome Segregation physiology
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
Female
HeLa Cells
Humans
Male
Microtubules metabolism
Microtubules ultrastructure
Oocytes cytology
Oocytes physiology
Protein Kinases genetics
Protein Serine-Threonine Kinases
Spermatozoa
Spindle Apparatus metabolism
Spindle Apparatus ultrastructure
Xenopus
Cell Nucleus metabolism
Centromere metabolism
Mitosis physiology
Protein Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 176
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 17389228
- Full Text :
- https://doi.org/10.1083/jcb.200609044