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NMR as a tool for elucidating the structures of circular and knotted proteins.
- Source :
-
Molecular bioSystems [Mol Biosyst] 2007 Apr; Vol. 3 (4), pp. 257-65. Date of Electronic Publication: 2007 Jan 25. - Publication Year :
- 2007
-
Abstract
- Cyclotides are a recently discovered family of mini-proteins that have a head-to-tail cyclised backbone stabilized by a knotted arrangement of three disulfide bonds. They have a wide range of biological activities, including uterotonic, anti-bacterial, anti-HIV, and anti-tumour activity but their insecticidal activities suggest that their natural function is in plant defense. They are exceptionally resistant to chemical, enzymatic and thermal treatments because of their unique structural scaffold. This stability and resistance to proteolysis makes them a potentially valuable protein engineering tool at the interface of chemistry and biology: they have the structure of proteins but the stability and biophysical properties of organic molecules. In this review the role of NMR in defining the structures of cyclotides is described.
- Subjects :
- Amino Acid Sequence
Cyclotides metabolism
Metals metabolism
Molecular Sequence Data
Phosphorylcholine analogs & derivatives
Phosphorylcholine metabolism
Protein Folding
Protein Structure, Secondary
Structure-Activity Relationship
Cyclotides chemistry
Cystine Knot Motifs
Nuclear Magnetic Resonance, Biomolecular methods
Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1742-206X
- Volume :
- 3
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecular bioSystems
- Publication Type :
- Academic Journal
- Accession number :
- 17372654
- Full Text :
- https://doi.org/10.1039/b616856f