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Gene expression analysis offers unique advantages to histopathology in liver biopsy evaluations.
- Source :
-
Toxicologic pathology [Toxicol Pathol] 2007 Feb; Vol. 35 (2), pp. 276-83. - Publication Year :
- 2007
-
Abstract
- Liver diseases that induce nonuniform lesions often give rise to greatly varying histopathology results in needle biopsy samples from the same patient. This study examines whether gene expression analysis of such biopsies could provide a more representative picture of the overall condition of the liver. We utilized acetaminophen (APAP) as a model hepatotoxicant that gives a multifocal pattern of necrosis following toxic doses. Rats were treated with a single toxic or subtoxic dose of APAP and sacrificed 6, 24, or 48 hours after exposure. Left liver lobes were harvested, and both gene expression and histopathological analysis were performed on biopsy-sized samples. While histopathological evaluation of such small samples revealed significant sample to sample differences after toxic doses of APAP, gene expression analysis provided a very homogeneous picture and allowed clear distinction between subtoxic and toxic doses. The main biological function differentiating animals that received sub-toxic from those that had received toxic doses was an acute stress response at 6 hours and signs of energy depletion at later time points. Our results suggest that the use of genomic analysis of biopsy samples together with histopathological analysis could provide a more precise representation of the overall condition of a patient's liver than histopathological evaluation alone.
- Subjects :
- Acetaminophen adverse effects
Analgesics, Non-Narcotic adverse effects
Animals
Biopsy
Cytochrome P-450 Enzyme System metabolism
Dose-Response Relationship, Drug
Liver drug effects
Liver Diseases complications
Male
Necrosis chemically induced
Necrosis metabolism
Necrosis pathology
Rats
Rats, Inbred F344
Gene Expression Profiling
Liver metabolism
Liver pathology
Liver Diseases genetics
Liver Diseases pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0192-6233
- Volume :
- 35
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Toxicologic pathology
- Publication Type :
- Academic Journal
- Accession number :
- 17366322
- Full Text :
- https://doi.org/10.1080/01926230601178207