Relationship between MPA free fraction and free MPAG concentrations in heart transplant recipients based on simultaneous HPLC quantification of the target compounds in human plasma.

Authors :: Cussonneau X
Bolon-Larger M
Prunet-Spano C
Bastien O
Boulieu R
Source :: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2007 Jun 01; Vol. 852 (1-2), pp. 674-8. Date of Electronic Publication: 2007 Feb 28.
Publication Year :: 2007
Abstract: A simple and sensitive HPLC method for the simultaneous analysis of free MPA and free MPAG was developed. Separation was achieved on a X-Terra RP18 column with acetonitrile-40 mM orthophosphoric acid as eluents using a gradient elution mode over 35 min at a flow rate of 1.5 ml/min. The assay was linear in the range 0.005 mg/L (LOQ) to 5mg/L for free MPA and 0.05 mg/L (LOQ) to 200 mg/L for free MPAG. Isolation of free MPA and free MPAG was done by ultrafiltration and the ultrafiltrate was directly injected. A positive correlation between MPA free fractions and free MPAG concentrations was found. Likewise, free MPAG was related to total MPAG concentrations in the seven heart transplant patients.

Subjects :: Glucuronides pharmacokinetics
Humans
Mycophenolic Acid pharmacokinetics
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Ultrafiltration
Chromatography, High Pressure Liquid methods
Glucuronides blood
Heart Transplantation
Mycophenolic Acid analogs & derivatives
Mycophenolic Acid blood

Language :: English
ISSN :: 1570-0232
Volume :: 852
Issue :: 1-2
Database :: MEDLINE
Journal :: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Publication Type :: Academic Journal
Accession number :: 17350903
Full Text :: https://doi.org/10.1016/j.jchromb.2007.02.038

Full Text Access

Tools