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[Quantitative detection of DD3 mRNA in prostate cancer tissues by real-time fluorescent quantitative reverse transcription polymerase chain reaction].
- Source :
-
Zhonghua nan ke xue = National journal of andrology [Zhonghua Nan Ke Xue] 2007 Feb; Vol. 13 (2), pp. 130-3. - Publication Year :
- 2007
-
Abstract
- Objective: To analyze the expression of DD3 mRNA in the prostate tissues.<br />Methods: DD3 mRNA was detected by realtime fluorescent quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR) based on the Taqman technique in the tissues of 27 patients with non-prostate cancer( NPCa), 21 prostate cancer( PCa), 39 benign prostatic hyperplasia (BPH) and 15 normal prostate (NP). The ROC curve was used to evaluate the diagnostic value of DD3 mRNA.<br />Results: DD3 mRNA expression was not detected in the NPCa tissues. The median expressions of DD3 mRNA in PCa, BPH and NP tissues were 7. 2 x 10(6), 2. 5 x 10(4) and 1.5 x 10(4) copies/mg tissue, respectively. The DD3 mRNA expression levels were significantly different between nonmalignant and malignant tissues (P < 0.01). No significant differences in DD3 mRNA expression were detected between the NP and BPH tissues and no significant correlation was found between the DD3 mRNA expression and clinical pathological parameters. The AUC-ROC was 0.937 (95% CI: 0.879 - 0.995) at cutoff value 1.4 x 10(5) copies/mg tissue. The sensitivity, specificity, accuracy, positive predictive value, negative predictive value, positive likelihood ratio and negative likelihood ratio for DD3 were 90.5%, 85.0%, 86.7%, 76.0%, 94.3%, 6.03 and 0.11 respectively.<br />Conclusion: The DD3 mRNA expression is confined to prostate tissues and highly upregulated in PCa tissues. It has a potential application value in the early diagnosis of prostate cancer and the follow-up of the patient.
- Subjects :
- Aged
Aged, 80 and over
Antigens, Neoplasm genetics
Humans
Male
Middle Aged
Neoplasm Staging
Prostatic Hyperplasia metabolism
Prostatic Hyperplasia pathology
Prostatic Neoplasms pathology
RNA, Messenger genetics
ROC Curve
Reverse Transcriptase Polymerase Chain Reaction
Antigens, Neoplasm biosynthesis
Prostatic Neoplasms metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1009-3591
- Volume :
- 13
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Zhonghua nan ke xue = National journal of andrology
- Publication Type :
- Academic Journal
- Accession number :
- 17345768