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Skin-homing CD8+ T lymphocytes show preferential growth in vitro and suppress CD4+ T-cell proliferation in patients with early stages of cutaneous T-cell lymphoma.
- Source :
-
Acta dermato-venereologica [Acta Derm Venereol] 2007; Vol. 87 (2), pp. 118-26. - Publication Year :
- 2007
-
Abstract
- A total of 27 T-lymphocyte cell strains were established from skin biopsies of 24 patients with various stages of cutaneous T-cell lymphoma (CTCL) by addition of the T-cell growth factors interleukin (IL)-2 and IL-4. Cellular proliferation and phenotypic changes were measured over 3 months in culture, and T-cell clones were studied using T-cell receptor-? re-arrangement techniques. An average outgrowth of 134 million T-lymphocytes from a 4-mm skin biopsy was observed over 2 months. Initially, most T-cells expressed the CD4+ phenotype. In 17 cell strains from patients with early CTCL a statistically significant predominance of CD8+ T-lymphocytes developed over 8-weeks' culture, indicating that CD8+ T-cells controlled the growth of CD4+ T cells, whereas CD4+ T-cells were predominant in cell strains from advanced CTCL (p <0.05). TCR-? re-arrangement studies revealed, on average, 12 T-cell clones per cell strain, which was reduced over time to 6 T-cell clones per cell strain. Lymphocytes from peripheral blood could kill lymphocytes from an autologous cell strain, suggesting the presence of autoreactive cytotoxic T-cells. Our study suggests how skin-homing CD8+ T-lymphocytes from patients with early stage CTCL can suppress the in vitro growth of skin-homing CD4+ T-lymphocytes, indicating immune surveillance.
- Subjects :
- Adolescent
Adult
Aged
CD4-Positive T-Lymphocytes pathology
CD8-Positive T-Lymphocytes pathology
Cell Cycle physiology
Female
Humans
Immunologic Surveillance
Interleukin-2 immunology
Interleukin-4 immunology
Lymphocyte Activation
Lymphoma, T-Cell, Cutaneous pathology
Male
Middle Aged
Neoplasm Staging
Receptors, Antigen, T-Cell, gamma-delta immunology
Skin Neoplasms pathology
X Chromosome Inactivation genetics
CD4-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes immunology
Lymphoma, T-Cell, Cutaneous immunology
Skin Neoplasms immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0001-5555
- Volume :
- 87
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Acta dermato-venereologica
- Publication Type :
- Academic Journal
- Accession number :
- 17340017
- Full Text :
- https://doi.org/10.2340/00015555-0206