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Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2007; Vol. 31 (9), pp. 859-73. Date of Electronic Publication: 2007 Feb 20. - Publication Year :
- 2007
-
Abstract
- The work presented here reports the expression of immune-related genes during ontogenesis in the oyster Crassostrea gigas. Expression patterns of 18 selected genes showed that RNAs detected in oocytes and 2-4 cell embryos are of maternal origin and that gene transcription starts early after fertilization. The expression patterns of 4 genes (Cg-timp, Cg-tal, Cg-EcSOD and Drac3) suggested that hemocytes appear in the gastrula-trochophore stages. The localization of Cg-tal expression suggested that hematopoietic cells were derived from vessels and/or artery endothelia cells. Moreover, a bacterial challenge affected the level of expression of genes. Indeed, a change in expression levels was observed for Cg-LBP/BPI, Cg-timp, Drac3 and Cg-MyD88 genes in larval stages upon exposure to non-pathogenic bacteria. In early juveniles, a modulation was also observed for Cg-LBP/BPI, Cg-timp, Cg-MyD88 and for Cg-tal, according to the concentration of bacteria. Altogether, the results showed that studying the appearance of immunocompetent cells through their ability to express immune-related genes is a tool to gain insight the ontogenesis of the oyster immune system.
- Subjects :
- Animal Diseases genetics
Animal Diseases microbiology
Animal Diseases pathology
Animals
Bacterial Infections genetics
Bacterial Infections microbiology
Bacterial Infections pathology
Crassostrea embryology
Crassostrea growth & development
Female
Male
RNA, Messenger genetics
Superoxide Dismutase genetics
Superoxide Dismutase metabolism
Tissue Inhibitor of Metalloproteinases genetics
Tissue Inhibitor of Metalloproteinases metabolism
Ubiquitin-Protein Ligases genetics
Ubiquitin-Protein Ligases metabolism
rac GTP-Binding Proteins genetics
Crassostrea genetics
Crassostrea immunology
Gene Expression Regulation genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0145-305X
- Volume :
- 31
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 17337052
- Full Text :
- https://doi.org/10.1016/j.dci.2007.01.005