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Growth inhibition and induction of apoptosis in NB4 promyelocytic leukemia cells by trypsin inhibitor from sweet potato storage roots.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2007 Apr 04; Vol. 55 (7), pp. 2548-53. Date of Electronic Publication: 2007 Mar 01. - Publication Year :
- 2007
-
Abstract
- The objective of this study was to investigate the antiproliferative effect and the mechanism of trypsin inhibitor (TI) from sweet potato [Ipomoea batatas (L.) Lam. 'Tainong 57'] storage roots on NB4 promyelocytic leukemia cells. The results showed that TI inhibited cellular growth of NB4 promyelocytic leukemia cells in a time-dependent and dose-dependent manner, and treatment for 72 h induced a marked inhibition of cellular growth, showing an IC50 of 57.1 +/- 8.26 microg/mL. TI caused cell cycle arrest at the G1 phase as determined by flow cytometric analysis and apoptosis as shown by DNA laddering. TI-induced cell apoptosis involved p53, Bcl-2, Bax, and cytochrome c protein in NB4 cells. P53 and Bax proteins were accumulated, and antiapoptotic molecule Bcl-2 was decreased in the tested cells in a time-dependent manner during TI treatment. TI also induced a substantial release of cytochrome c from the mitochondria into the cytosol. Hence, TI induced apoptosis in NB4 cells through a mitochondria-dependent pathway, which was associated with the activation of caspase-3 and -8. These results demonstrated that TI induces NB4 cell apoptosis through the inhibition of cell growth and the activation of the pathway of caspase-3 and -8 cascades.
- Subjects :
- Caspases metabolism
Cell Cycle Proteins analysis
Cell Line, Tumor
DNA Fragmentation drug effects
Flow Cytometry
Humans
Leukemia, Promyelocytic, Acute
Trypsin Inhibitors isolation & purification
Apoptosis drug effects
Cell Division drug effects
Ipomoea batatas chemistry
Plant Tubers chemistry
Trypsin Inhibitors pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-8561
- Volume :
- 55
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17328557
- Full Text :
- https://doi.org/10.1021/jf063008m