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Multicenter evaluation of a rapid electrochemiluminescent adrenocorticotropic hormone (ACTH) immunoassay.
- Source :
-
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2007 May 01; Vol. 380 (1-2), pp. 75-80. Date of Electronic Publication: 2007 Jan 24. - Publication Year :
- 2007
-
Abstract
- Background: Measuring plasma adrenocorticotropic hormone (ACTH) is a key step in the differential diagnosis of hypothalamic-pituitary-adrenal disorders.<br />Methods: The recently developed electrochemiluminescence Elecsys ACTH immunoassay (Roche Diagnostics, Mannheim, Germany) was evaluated at six clinical laboratories on the Modular E170 and/or the Elecsys 2010 (Roche Diagnostics) immunoanalysers.<br />Results: The within-run and between-run imprecision was <or=1.9% and <or=5%, respectively (concentration level 12-971 ng/L). Functional sensitivity was at least 1.8 ng/L. The Elecsys assay correlated well with two established ACTH immunoradiometric assays (Brahms ACTH RIA and Nichols ACTH IRMA) and with the Immulite ACTH assay. Reference values for the Elecsys ACTH assay, determined as the percentile 5 to 95 (2.5 to 97.5) interval in 354 adults without disorder with impact on the hypothalamic-pituitary-adrenal axis, were between 7.2 and 63 ng/L (5.6 and 76 ng/L). The clinical sensitivity of the Elecsys ACTH assay in 57 patients with confirmed disorders of the pituitary-adrenal axis was comparable to the established Brahms ACTH assay.<br />Conclusion: The non-isotopic Elecsys ACTH immunoassay provides precision and reliability in combination with reduced turnaround time.
- Subjects :
- Adrenal Gland Diseases blood
Adult
Aged
Aged, 80 and over
Female
Humans
Immunoassay instrumentation
Immunoassay statistics & numerical data
Luminescent Measurements instrumentation
Luminescent Measurements statistics & numerical data
Male
Middle Aged
Pituitary Diseases blood
Pituitary-Adrenal System metabolism
Pituitary-Adrenal System pathology
Reference Values
Sensitivity and Specificity
Adrenal Gland Diseases diagnosis
Adrenocorticotropic Hormone analysis
Immunoassay methods
Luminescent Measurements methods
Pituitary Diseases diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0009-8981
- Volume :
- 380
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Clinica chimica acta; international journal of clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17321508
- Full Text :
- https://doi.org/10.1016/j.cca.2006.12.031