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Human keratinocytes produce the complement inhibitor factor I: Synthesis is regulated by interferon-gamma.
- Source :
-
Molecular immunology [Mol Immunol] 2007 Apr; Vol. 44 (11), pp. 2943-9. Date of Electronic Publication: 2007 Feb 22. - Publication Year :
- 2007
-
Abstract
- Extrahepatic complement synthesis is believed to play an important role in host defense and inflammation at tissue and organ level. In the epidermis the most abundant cell type, keratinocytes have been shown to produce C3, factor B and factor H. In the present study, we investigated the synthesis of factor I by human keratinocytes. We also studied whether proinflammatory cytokines IL-1alpha, IL-6, TGF-beta1, TNF-alpha and IFN-gamma regulate factor I synthesis in keratinocytes. Human keratinocytes constitutively expressed factor I mRNA and produced factor I protein. Amongst the above-mentioned cytokines, only IFN-gamma regulated the synthesis of factor I, and this effect occurred predominantly at pre-translational level. Factor I produced by keratinocytes was functionally active in cleaving C3b. In conclusion, we demonstrate that keratinocytes are capable of synthesizing factor I, and that this synthesis is regulated by IFN-gamma.
- Subjects :
- Cells, Cultured
Complement Factor I genetics
Cytokines pharmacology
Gene Expression Regulation drug effects
Humans
Protein Biosynthesis drug effects
RNA, Messenger biosynthesis
RNA, Messenger genetics
Recombinant Proteins
Complement Factor I biosynthesis
Interferon-gamma pharmacology
Keratinocytes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0161-5890
- Volume :
- 44
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 17320177
- Full Text :
- https://doi.org/10.1016/j.molimm.2007.01.007