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Expression of the G protein gammaT1 subunit during zebrafish development.
- Source :
-
Gene expression patterns : GEP [Gene Expr Patterns] 2007 Apr; Vol. 7 (5), pp. 574-83. Date of Electronic Publication: 2007 Jan 17. - Publication Year :
- 2007
-
Abstract
- Here, we report the identification and expression analysis of the zebrafish G protein gammaT1 subunit gene (gngT1) during development. Similar to its human and mouse homologs, we confirm zebrafish gngT1 is expressed in the developing retina, where its transcription overlaps with the photoreceptor cell-specific marker, rhodopsin (rho). Surprisingly, we also show zebrafish gngT1 is expressed in the dorsal diencephalon, where its transcription overlaps with the pineal specific markers, arylalkylamine N-acetyltransferase-2 (annat-2) and extra-ocular rhodopsin (exorh). Analysis of the proximal promoter sequence of the zebrafish gngT1 gene identifies several conserved binding sites for the cone-rod homeobox/orthodenticle (Crx/Otx) homeodomain family of transcription factors. Using a morpholino anti-sense approach in zebrafish, we show that targeted knockdown of otx5 potently suppresses gngT1 expression in the pineal gland, whereas knockdown of crx markedly reduces gngT1 expression in the retina. Taken together, these data indicate that pineal- and retinal-specific expression of the gngT1 gene are controlled by different transcription factors and exogenous signals.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
GTP-Binding Protein gamma Subunits genetics
Homeodomain Proteins genetics
Homeodomain Proteins metabolism
In Situ Hybridization
Molecular Sequence Data
Oligonucleotides, Antisense pharmacology
Otx Transcription Factors genetics
Otx Transcription Factors metabolism
Photoreceptor Cells metabolism
Pineal Gland cytology
Pineal Gland metabolism
RNA Probes
Retina cytology
Retina metabolism
Rhodopsin metabolism
Sequence Homology, Amino Acid
Trans-Activators genetics
Trans-Activators metabolism
Zebrafish growth & development
Zebrafish metabolism
Zebrafish Proteins genetics
GTP-Binding Protein gamma Subunits metabolism
Gene Expression Regulation, Developmental
Zebrafish genetics
Zebrafish Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1567-133X
- Volume :
- 7
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Gene expression patterns : GEP
- Publication Type :
- Academic Journal
- Accession number :
- 17306630
- Full Text :
- https://doi.org/10.1016/j.modgep.2007.01.003