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Purification of an Abf2p-like protein from mitochondrial nucleoids of yeast Pichia jadinii and its role in the packaging of mitochondrial DNA.
- Source :
-
Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2007 Apr; Vol. 91 (3), pp. 197-207. Date of Electronic Publication: 2007 Feb 13. - Publication Year :
- 2007
-
Abstract
- A 26-kDa protein with highly basic pI was purified from the mitochondrial (mt-) nucleoids of the yeast Pichia jadinii by a combination of acid extraction, hydroxyapatite chromatography and DNA-cellulose chromatography. The 26-kDa protein has the ability to introduce a supercoil into circular plasmid DNA in the presence of topoisomerase I and to package mtDNA into nucleoid-like aggregates. The mt-nucleoids isolated from P. jadinii cells were disassembled in the presence of 2 M NaCl and reassembled into nucleoid-like aggregates by the removal of the salts. During the course of the reassembly of the mt-nucleoids, three specific proteins of 20 kDa, 26 kDa and 56 kDa predominantly precipitated after the centrifugation of the reassembled mt-nucleoids. These results suggest that the 26-kDa protein of P. jadinii has a similar function in the packaging of mtDNA to Abf2p, a major mitochondrial DNA-binding protein in Saccharomyces cerevisiae.
Details
- Language :
- English
- ISSN :
- 0003-6072
- Volume :
- 91
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Antonie van Leeuwenhoek
- Publication Type :
- Academic Journal
- Accession number :
- 17295092
- Full Text :
- https://doi.org/10.1007/s10482-006-9105-7