Back to Search
Start Over
Monoclonal antibodies to idiotype inhibit in vitro growth of human B-cell lymphomas.
- Source :
-
Blood [Blood] 1992 Jan 01; Vol. 79 (1), pp. 129-37. - Publication Year :
- 1992
-
Abstract
- The effect of monoclonal antibodies (MoAbs) recognizing idiotype, IgM heavy chain, and IgD heavy chain on the in vitro DNA synthesis of five randomly selected leukemic human low-malignancy B-cell lymphomas was investigated. In three lymphomas of different histologic subtype, low concentrations of anti-idiotypic (anti-Id) MoAb completely inhibited spontaneous 3H-thymidine uptake of T-cell-- and monocyte-depleted tumor cells, whereas two other tumors were not affected. Maximal inhibition of DNA synthesis was achieved at MoAb concentrations ranging from 0.5 to 250 micrograms/mL and required crosslinking by bivalent antibody but not Fc-mediated effects. While two anti-IgM MoAbs were similarly efficient as anti-Id MoAb in inhibition of DNA synthesis, two anti-IgD MoAbs had no effect. Thus, surface IgD molecules seemed to be neither able to deliver inhibitory signals themselves nor to antagonize IgM-mediated signals when simultaneously crosslinked by anti-Id MoAb. Leukocyte differentiation antigen expression, IgM density, and IgM/IgD ratio on the surface of lymphoma cells did not distinguish between sensitive and resistant tumors. In vitro tumor cell survival was differently affected by prolonged incubation with anti-Id antibody. In a centrocytic lymphoma and an immunocytoma, but not in a chronic lymphocytic leukemia, suppression of 3H-thymidine uptake persisted after removal of MoAb and tumor cell viability decreased during prolonged incubation with anti-Id MoAb. These results suggest that direct inhibitory signaling via surface IgM may contribute to anti-Id MoAb-mediated tumor regression in certain human B-cell lymphomas.
- Subjects :
- Cell Survival
Cross-Linking Reagents
DNA, Neoplasm biosynthesis
Humans
Immunoglobulin D immunology
Immunoglobulin Heavy Chains immunology
Immunoglobulin M immunology
Immunophenotyping
Lymphoma, B-Cell immunology
Lymphoma, B-Cell metabolism
Phytohemagglutinins
Receptors, Fc immunology
Antibodies, Monoclonal
Antigens, Neoplasm immunology
Immunoglobulin Idiotypes immunology
Lymphoma, B-Cell pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 79
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 1728304