Back to Search
Start Over
Expression patterns of inhibitor of apoptosis proteins in malignant pleural mesothelioma.
- Source :
-
The Journal of pathology [J Pathol] 2007 Mar; Vol. 211 (4), pp. 447-54. - Publication Year :
- 2007
-
Abstract
- Inhibitor of apoptosis proteins (IAPs) comprise a family of structurally similar proteins, five of which are widely studied in the context of cancer: IAP-1/MIHC/cIAP2, IAP-2/MIHB/cIAP1, livin/ML-IAP/KIAP, survivin, and XIAP/MIHA/hILP. IAPs are overexpressed by most neoplasms, promote tumour cell survival after a wide variety of apoptotic stimuli, and frequently have gene and/or protein expression patterns associated with a relatively poor prognosis. However, many IAPs are also expressed by normal tissues, can facilitate apoptotic cell death, and have expression patterns associated with a relatively favourable prognosis in some cases. The result is that the precise role(s) of IAPs in human tumours is not exactly known. It has been previously reported that IAP-1 is overexpressed in malignant pleural mesothelioma (MPM) and is responsible for a large degree of the resistance of cultured MPM cells to cisplatin. Given the high homology of IAP family members, it is likely that other IAPs will be important in MPM. In the present study, the gene and protein expression patterns of IAP-1, IAP-2, survivin, livin, and XIAP have been determined in MPM cell lines (n=9) and a large number of MPM tumours using high-density oligonucleotide microarrays (n=40) and an MPM tissue array (n=66). Human tumours were linked to clinical data and it was found that IAP-1 and survivin mRNA expression patterns were associated with a relatively shorter patient survival, while those of XIAP and livin were associated with a relatively longer patient survival. Abundant protein for all IAPs was also detected in MPM tumours, where they were expressed primarily in the cytoplasm. Only IAP-1 and livin protein was expressed in the nucleus of MPM tumours. These results provide the rationale for additional study of this gene family in MPM and cancer in general.<br /> (Copyright (c) 2007 Pathological Society of Great Britain and Ireland.)
- Subjects :
- Adaptor Proteins, Signal Transducing analysis
Cell Line, Tumor
Gene Expression Regulation, Neoplastic genetics
Humans
Immunohistochemistry methods
Kaplan-Meier Estimate
Mesothelioma mortality
Microtubule-Associated Proteins analysis
Pleural Neoplasms mortality
Prognosis
RNA, Messenger analysis
RNA, Neoplasm analysis
Survivin
X-Linked Inhibitor of Apoptosis Protein analysis
Inhibitor of Apoptosis Proteins analysis
Mesothelioma genetics
Neoplasm Proteins analysis
Pleural Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3417
- Volume :
- 211
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 17253596
- Full Text :
- https://doi.org/10.1002/path.2121