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[Study the value of screening hereditary nonpolyposis colorectal cancer kindreds by detecting the expression of hMLH1/hMSH2 with tissue microarray].
- Source :
-
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery [Zhonghua Wei Chang Wai Ke Za Zhi] 2007 Jan; Vol. 10 (1), pp. 67-9. - Publication Year :
- 2007
-
Abstract
- Objective: To study the value of screening hereditary nonpolyposis colorectal cancer (HNPCC) kindreds by detecting the expressions of hMLH1/hMSH2 with tissue microarray.<br />Methods: A tissue microarray with 22 colorectal cancers from HNPCC families and 15 sporadic colorectal cancers was established, and the expressions of hMLH1/hMSH2 were detected by immunohistochemistry (IHC).<br />Results: The expressions of hMLH1 or hMSH2 were negative in 15 of 22 HNPCC and 1 of 15 sporadic colorectal cancers in routine IHC. The expressions of hMLH1 or hMSH2 were negative in 17 of 22 HNPCC and 2 of 15 sporadic colorectal cancers in tissue microarray. The examination of hMSH2 expression yielded same results between routine IHC and tissue microarray. There were no difference on the hMLH1 expressions between routine IHC and tissue microarray.<br />Conclusion: Tissue microarray is a high-throughput way to detect the expressions of hMLH1/hMSH2 and is applicable to screen HNPCC kindreds.
- Subjects :
- Adaptor Proteins, Signal Transducing metabolism
Adult
Aged
Colorectal Neoplasms, Hereditary Nonpolyposis metabolism
DNA Methylation
Female
Gene Frequency
Genetic Testing
Humans
Immunohistochemistry
Male
Middle Aged
MutL Protein Homolog 1
MutS Homolog 2 Protein metabolism
Nuclear Proteins metabolism
Pedigree
Colorectal Neoplasms, Hereditary Nonpolyposis diagnosis
Colorectal Neoplasms, Hereditary Nonpolyposis genetics
Protein Array Analysis methods
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1671-0274
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery
- Publication Type :
- Academic Journal
- Accession number :
- 17253179