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Certification of methylmercury content in two fresh-frozen reference materials: SRM 1947 Lake Michigan fish tissue and SRM 1974b organics in mussel tissue (Mytilus edulis).
- Source :
-
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2007 Apr; Vol. 387 (7), pp. 2335-41. Date of Electronic Publication: 2007 Jan 23. - Publication Year :
- 2007
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Abstract
- This paper describes the development of two independent analytical methods for the extraction and quantification of methylmercury from marine biota. The procedures involve microwave extraction, followed by derivatization and either headspace solid-phase microextraction (SPME) with a polydimethylsiloxane (PDMS)-coated silica fiber or back-extraction into iso-octane. The identification and quantification of the extracted compounds is carried out by capillary gas chromatography/mass spectrometric (GC/MS) and inductively coupled plasma mass spectrometric (GC/ICP-MS) detection. Both methods were validated for the determination of methylmercury (MeHg) concentrations in a variety of biological standard reference materials (SRMs) including fresh-frozen tissue homogenates of SRM 1946 Lake Superior fish tissue and SRM 1974a organics in mussel tissue (Mytilus edulis) and then applied to the certification effort of SRM 1947 Lake Michigan fish tissue and SRM 1974b organics in mussel tissue (Mytilus edulis). While past certifications of methylmercury in tissue SRMs have been based on two independent methods from the National Institute of Standards and Technology (NIST) and participating laboratories, the methods described within provide improved protocols and will allow future certification efforts to be based on at least two independent analytical methods within NIST.
- Subjects :
- Animals
Chromatography, Gas methods
Fishes
Food Contamination
Freezing
Gas Chromatography-Mass Spectrometry methods
Meat analysis
Reference Values
Solid Phase Microextraction methods
United States
Methylmercury Compounds analysis
Mytilus edulis chemistry
Seafood analysis
Trace Elements analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1618-2642
- Volume :
- 387
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Analytical and bioanalytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17242887
- Full Text :
- https://doi.org/10.1007/s00216-006-1106-x