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Enhanced protection against Streptococcus pyogenes infection by intranasal vaccination with a dual antigen component M protein/SfbI lipid core peptide vaccine formulation.
- Source :
-
Vaccine [Vaccine] 2007 Feb 26; Vol. 25 (10), pp. 1789-97. Date of Electronic Publication: 2006 Nov 30. - Publication Year :
- 2007
-
Abstract
- We investigated the efficacy of a synthetic Streptococcus pyogenes vaccine targeting two virulence factors using the Lipid Core Peptide (LCP) delivery system. BALB/c mice were immunised intranasally with LCPs containing peptides encompassing T-cell and B-cell epitopes of the conserved C-repeat region of the M protein (J8) or the fibronectin-binding repeats region (FNBR) of SfbI, or a combination formulation containing peptides representing both antigens. LCPs were co-administered with the TLR2/6 agonist MALP-2 as mucosal adjuvant. Humoral and cellular immune responses stimulated at systemic and mucosal levels were strongest in mice immunised with the dual antigen formulation. Mice were completely protected following a respiratory challenge with a lethal dose of a heterologous S. pyogenes strain, whereas there was 70% and 90% survival in mice immunised with LCP-J8 and LCP-FNBR, respectively. This is the first report demonstrating the elicitation of better protective immunity by a dual antigen component S. pyogenes vaccine.
- Subjects :
- Adhesins, Bacterial genetics
Adjuvants, Immunologic administration & dosage
Adjuvants, Immunologic pharmacology
Administration, Intranasal
Animals
Antibodies, Bacterial blood
Antigens, Bacterial genetics
Bacterial Outer Membrane Proteins genetics
Carrier Proteins genetics
Disease Models, Animal
Epitopes, B-Lymphocyte genetics
Epitopes, B-Lymphocyte immunology
Epitopes, T-Lymphocyte genetics
Epitopes, T-Lymphocyte immunology
Leukocytes, Mononuclear immunology
Lipopeptides
Mice
Mice, Inbred BALB C
Oligopeptides administration & dosage
Oligopeptides pharmacology
Streptococcal Infections immunology
Streptococcal Vaccines administration & dosage
Streptococcus pyogenes genetics
Vaccines, Subunit administration & dosage
Vaccines, Subunit immunology
Vaccines, Synthetic administration & dosage
Vaccines, Synthetic immunology
Adhesins, Bacterial immunology
Antigens, Bacterial immunology
Bacterial Outer Membrane Proteins immunology
Carrier Proteins immunology
Streptococcal Infections prevention & control
Streptococcal Vaccines immunology
Streptococcus pyogenes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 25
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 17229503
- Full Text :
- https://doi.org/10.1016/j.vaccine.2006.11.031