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Identification and characterisation of tomato torrado virus, a new plant picorna-like virus from tomato.
- Source :
-
Archives of virology [Arch Virol] 2007; Vol. 152 (5), pp. 881-90. Date of Electronic Publication: 2007 Jan 18. - Publication Year :
- 2007
-
Abstract
- A new virus was isolated from tomato plants from the Murcia region in Spain which showed symptoms of 'torrado disease'; very distinct necrotic, almost burn-like symptoms on leaves of infected plants. The virus particles are isometric with a diameter of approximately 28 nm. The viral genome consists of two (+)ssRNA molecules of 7793 (RNA1) and 5389 nts (RNA2). RNA1 contains one open reading frame (ORF) encoding a predicted polyprotein of 241 kDa that shows conserved regions with motifs typical for a protease-cofactor, a helicase, a protease and an RNA-dependent RNA polymerase. RNA2 contains two, partially overlapping ORFs potentially encoding proteins of 20 and 134 kDa. These viral RNAs are encapsidated by three proteins with estimated sizes of 35, 26 and 23 kDa. Direct protein sequencing mapped these coat proteins to ORF2 on RNA2. Phylogenetic analyses of nucleotide and derived amino acid sequences showed that the virus is related to but distinct from viruses belonging to the genera Sequivirus, Sadwavirus and Cheravirus. This new virus, for which the name tomato torrado virus is proposed, most likely represents a member of a new plant virus genus.
- Subjects :
- Amino Acid Motifs
Amino Acid Sequence
Capsid Proteins chemistry
Capsid Proteins genetics
Molecular Sequence Data
Molecular Weight
Open Reading Frames genetics
Phylogeny
Picornaviridae classification
Picornaviridae genetics
Polyproteins chemistry
Polyproteins genetics
Sequence Alignment
Spain
Species Specificity
Genome, Viral
Solanum lycopersicum virology
Picornaviridae isolation & purification
Plant Diseases virology
Subjects
Details
- Language :
- English
- ISSN :
- 0304-8608
- Volume :
- 152
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 17226066
- Full Text :
- https://doi.org/10.1007/s00705-006-0917-6