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Unexpected regioselectivity of Humicola insolens Cel7B glycosynthase mutants.
- Source :
-
Carbohydrate research [Carbohydr Res] 2007 Apr 09; Vol. 342 (5), pp. 710-6. Date of Electronic Publication: 2006 Dec 22. - Publication Year :
- 2007
-
Abstract
- Four Humicola insolens Cel7B glycoside hydrolase mutants have been evaluated for the coupling of lactosyl fluoride on O-allyl N(I)-acetyl-2(II)-azido-beta-chitobioside. Double mutants Cel7B E197A H209A and Cel7B E197A H209G preferentially catalyze the formation of a beta-(1-->4) linkage between the two disaccharides, while single mutant Cel7B E197A and triple mutant Cel7B E197A H209A A211T produce predominantly the beta-(1-->3)-linked tetrasaccharide. This result constitutes the first report of the modulation of the regioselectivity through site-directed mutagenesis for an endoglycosynthase.
- Subjects :
- Ascomycota genetics
Ascomycota metabolism
Azides chemical synthesis
Azides chemistry
Carbohydrate Sequence
Catalysis
Chromatography, High Pressure Liquid
Disaccharides chemical synthesis
Disaccharides chemistry
Disaccharides metabolism
Glycoside Hydrolases chemistry
Glycosides biosynthesis
Glycosides chemistry
Lactose analogs & derivatives
Lactose chemistry
Mutagenesis, Site-Directed
Oligosaccharides chemistry
Oligosaccharides metabolism
Stereoisomerism
Ascomycota enzymology
Glycoside Hydrolases genetics
Glycoside Hydrolases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0008-6215
- Volume :
- 342
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Carbohydrate research
- Publication Type :
- Academic Journal
- Accession number :
- 17224137
- Full Text :
- https://doi.org/10.1016/j.carres.2006.12.017