[Influence of bifidobacterium DNA on PKC and NF-kappaB in murine macrophages].

Aim: To explore the influence of DNA of bifidobacteriua adolescence on PKC and NK-kappaB of murine macrophages.
Methods: The fluorescent intensity of PKCalpha, PKCbetaI, PKCbetaII, PKCgamma, PKCepsilon and PKCzeta in murine peritoneal macrophages was detected by using laser confocal microscope. The density of NK-kappaB(+) macrophages was detected by immunocytochemical staining.
Results: The average fluorescent intensity of PKCalpha and PKCbetaII produced by mouse peritoneal macrophages in bifidobacterium DNA injection group was markedly higher than that in control group(P<0.01). There was no statistically significant difference of average fluorescent intensity of PKCbetaI, PKCgamma, PKCepsilon and PKCzeta between the two groups (P>0.05). The density of NK-kappaB(+) macrophages in bifidobacterium DNA injection group was markedly higher than that in control group (P<0.01).
Conclusion: DNA of bifidobacteria adolescence could activate macrophages by promoting the activity of PKCalpha, PKCbetaII and NF-kappaB.