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Babesia bovis: subcellular localization of host erythrocyte membrane components during their asexual growth.

Babesia bovis: subcellular localization of host erythrocyte membrane components during their asexual growth.

Authors :
Okamura M
Yokoyama N
Takabatake N
Okubo K
Ikehara Y
Igarashi I
Source :
Experimental parasitology [Exp Parasitol] 2007 May; Vol. 116 (1), pp. 91-4. Date of Electronic Publication: 2007 Jan 04.
Publication Year :
2007

Abstract

In the present study, the subcellular localization of the host red blood cell (RBC) membrane components, the alpha2-3-linked sialic acid (SA) residues and the lipid bilayer, was observed during the asexual growth of Babesia bovis using two erythrocyte probes, the SA-specific lectin (MALII) and the lipophilic fluorescent (PKH2) probes, respectively. In confocal laser scanning microscopy with MALII, the SA residues on the surface of parasitized RBCs appeared to accumulate into the intracellular parasites as the parasites matured as well as to remain on the surface of extracellular parasites. Furthermore, when PKH2-labeled RBCs were infected with B. bovis, PKH2 signals were also observed around both the intracellular and the extracellular parasites, similarly to the results of MALII. These results indicated that the components derived from the host erythrocyte membrane are incorporated into the intracellular parasites during their asexual growth within the parasitized RBC, suggesting the possible formation of a parasitophorous vacuole-based network or a parasite surface coat.

Details

Language :
English
ISSN :
0014-4894
Volume :
116
Issue :
1
Database :
MEDLINE
Journal :
Experimental parasitology
Publication Type :
Academic Journal
Accession number :
17207481
Full Text :
https://doi.org/10.1016/j.exppara.2006.11.006