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Isolation and expression of a guanylate cyclase-coupled heat stable enterotoxin receptor cDNA from a human colonic cell line.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1991 Sep 30; Vol. 179 (3), pp. 1455-63. - Publication Year :
- 1991
-
Abstract
- Heat stable enterotoxins (STs) are low molecular-weight peptides secreted by enterotoxigenic bacteria. One type of these enterotoxins (STa) induces intestinal secretion leading to acute diarrhea by binding to a membrane form of guanylate cyclase. We have isolated a cDNA from a human colonic cell line, T84, encoding for a guanylate cyclase-coupled enterotoxin receptor (STaR). The predicted amino acid sequence of the human STa receptor is 81% identical with the previously cloned enterotoxin receptor (GC-C) from rat intestine. COS-7 cells transiently transfected with the cloned cDNA expressed specific concentration-dependent response to STa as measured by cyclic GMP accumulation and is about 20 times more sensitive to the stimulation by STa than has been shown for GC-C.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cell Line
Colon
Cyclic GMP metabolism
DNA isolation & purification
Gene Library
Guanylate Cyclase metabolism
Humans
Kinetics
Molecular Sequence Data
RNA genetics
RNA isolation & purification
Rats
Receptors, Cell Surface metabolism
Receptors, Enterotoxin
Receptors, Guanylate Cyclase-Coupled
Sequence Homology, Nucleic Acid
Transfection
DNA genetics
Guanylate Cyclase genetics
Receptors, Cell Surface genetics
Receptors, Peptide
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 179
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 1718270
- Full Text :
- https://doi.org/10.1016/0006-291x(91)91736-v