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Detection of Streptococcus species by polymerase chain reaction in infectious crystalline keratopathy.

Authors :
Osakabe Y
Yaguchi C
Miyai T
Miyata K
Mineo S
Nakamura M
Amano S
Source :
Cornea [Cornea] 2006 Dec; Vol. 25 (10), pp. 1227-30.
Publication Year :
2006

Abstract

Purpose: Investigation of a positive polymerase chain reaction (PCR) for Streptococcus species from an excised cornea of a patient with infectious crystalline keratopathy.<br />Methods: Samples microdissected from corneal intrastromal aggregates present in infectious crystalline keratopathy were subjected to PCR for 3 primer pairs respectively specific for Streptococcus, Staphylococcus, and several kinds of fungi.<br />Results: The PCR detected DNA from Streptococcus but not from Staphylococcus or fungi. Direct sequencing of the PCR products showed that the PCR products had the highest identity (99.8%) with Streptococcus mitis and Streptococcus gordoni, indicating that these species were the most probable causes of infectious crystalline keratopathy in this patient.<br />Conclusion: Our findings suggest that PCR provides a good adjunct technique in the diagnosis of corneal infection.

Details

Language :
English
ISSN :
0277-3740
Volume :
25
Issue :
10
Database :
MEDLINE
Journal :
Cornea
Publication Type :
Academic Journal
Accession number :
17172904
Full Text :
https://doi.org/10.1097/01.ico.0000240083.31432.70