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Detection of Streptococcus species by polymerase chain reaction in infectious crystalline keratopathy.
- Source :
-
Cornea [Cornea] 2006 Dec; Vol. 25 (10), pp. 1227-30. - Publication Year :
- 2006
-
Abstract
- Purpose: Investigation of a positive polymerase chain reaction (PCR) for Streptococcus species from an excised cornea of a patient with infectious crystalline keratopathy.<br />Methods: Samples microdissected from corneal intrastromal aggregates present in infectious crystalline keratopathy were subjected to PCR for 3 primer pairs respectively specific for Streptococcus, Staphylococcus, and several kinds of fungi.<br />Results: The PCR detected DNA from Streptococcus but not from Staphylococcus or fungi. Direct sequencing of the PCR products showed that the PCR products had the highest identity (99.8%) with Streptococcus mitis and Streptococcus gordoni, indicating that these species were the most probable causes of infectious crystalline keratopathy in this patient.<br />Conclusion: Our findings suggest that PCR provides a good adjunct technique in the diagnosis of corneal infection.
- Subjects :
- Aged
Corneal Diseases pathology
Corneal Diseases surgery
Corneal Stroma microbiology
Corneal Stroma ultrastructure
Eye Infections, Bacterial pathology
Eye Infections, Bacterial surgery
Female
Humans
Keratoplasty, Penetrating
Polymerase Chain Reaction
Streptococcal Infections pathology
Streptococcal Infections surgery
Streptococcus mitis genetics
Bacterial Typing Techniques
Corneal Diseases microbiology
DNA, Bacterial analysis
Eye Infections, Bacterial microbiology
Streptococcal Infections microbiology
Streptococcus mitis classification
Subjects
Details
- Language :
- English
- ISSN :
- 0277-3740
- Volume :
- 25
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Cornea
- Publication Type :
- Academic Journal
- Accession number :
- 17172904
- Full Text :
- https://doi.org/10.1097/01.ico.0000240083.31432.70