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Classical nuclear localization signals: definition, function, and interaction with importin alpha.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2007 Feb 23; Vol. 282 (8), pp. 5101-5. Date of Electronic Publication: 2006 Dec 14. - Publication Year :
- 2007
-
Abstract
- The best understood system for the transport of macromolecules between the cytoplasm and the nucleus is the classical nuclear import pathway. In this pathway, a protein containing a classical basic nuclear localization signal (NLS) is imported by a heterodimeric import receptor consisting of the beta-karyopherin importin beta, which mediates interactions with the nuclear pore complex, and the adaptor protein importin alpha, which directly binds the classical NLS. Here we review recent studies that have advanced our understanding of this pathway and also take a bioinformatics approach to analyze the likely prevalence of this system in vivo. Finally, we describe how a predicted NLS within a protein of interest can be confirmed experimentally to be functionally important.
- Subjects :
- Active Transport, Cell Nucleus physiology
Animals
Humans
Nuclear Localization Signals genetics
Nuclear Pore genetics
alpha Karyopherins genetics
beta Karyopherins genetics
Nuclear Localization Signals metabolism
Nuclear Pore metabolism
alpha Karyopherins metabolism
beta Karyopherins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 282
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17170104
- Full Text :
- https://doi.org/10.1074/jbc.R600026200