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Acute myeloid leukemias with chromosomal abnormalities involving the 21q22 region identified by their in vitro responsiveness to interleukin-5.
- Source :
-
Leukemia [Leukemia] 1991 Aug; Vol. 5 (8), pp. 687-92. - Publication Year :
- 1991
-
Abstract
- Among 52 patients diagnosed as acute myeloid leukemia (AML), nine cases were found in which interleukin-5 (IL-5) induced a proliferative response in the leukemic cells, as measured by the stimulation of DNA synthesis or colony formation in vitro. All cases (n = 7) with the cytogenetic abnormality t(8;21)(q22;q22) belonged to this group of IL-5 responders. Of the additional two cases, one had an apparently normal karyotype, but the other expressed a dicentric chromosome 21, an abnormality also involving the breakpoint region 21q22. The leukemic cells of the IL-5 responsive patients could also be stimulated to proliferate by IL-3, GM-CSF and G-CSF, and in some cases by IL-6 or M-CSF. Immunophenotypic analysis revealed the presence of the immature hematopoietic cell antigen CD34, the myelomonocytic maturation antigens CD13 and CD33, in association with the B-cell related surface marker CD19 on the leukemic cells. Immunoglobulin mu and T-cell receptor beta-genes in the leukemic cells were in germline configuration. Upon incubation in colony culture, clonogenic cells were capable of producing progeny showing eosinophilic or neutrophilic maturation following stimulation with IL-5 or G-CSF, respectively. It is concluded that IL-5 responsive AML represents a subgroup of leukemia with distinct immunotypic and cytogenetic features.
- Subjects :
- Antigens, CD analysis
Cell Division drug effects
Chromosome Aberrations genetics
Chromosome Aberrations immunology
Chromosome Aberrations pathology
Chromosome Disorders
Chromosomes, Human, Pair 21
Female
Gene Rearrangement, B-Lymphocyte
Gene Rearrangement, T-Lymphocyte
Genes, Immunoglobulin
Granulocyte Colony-Stimulating Factor pharmacology
Granulocyte-Macrophage Colony-Stimulating Factor pharmacology
Humans
In Vitro Techniques
Interleukin-3 pharmacology
Leukemia, Myeloid immunology
Leukemia, Myeloid pathology
Male
Receptors, Antigen, T-Cell genetics
Tumor Cells, Cultured
Interleukin-5 pharmacology
Leukemia, Myeloid genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0887-6924
- Volume :
- 5
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Leukemia
- Publication Type :
- Academic Journal
- Accession number :
- 1715959