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Use of filter paper (FTA) technology for sampling, recovery and molecular characterisation of rabies viruses.
- Source :
-
Journal of virological methods [J Virol Methods] 2007 Mar; Vol. 140 (1-2), pp. 174-82. Date of Electronic Publication: 2006 Dec 06. - Publication Year :
- 2007
-
Abstract
- This study evaluates the feasibility of the use of the FTA Gene Guard System (a commercial product consisting of filter paper impregnated with patented chemicals supplied by the Whatman company) for the shipment, storage and detection of RNA rabies viruses by a simplified hemi-nested reverse transcriptase polymerase chain reaction. HnRT-PCR of the rabies virus nucleoprotein gene with specific primers showed that viral RNA extracted from crude infected tissues remained stable after fixation on the filter paper under diverse environmental conditions for at least 35 days. The sequence analysis of the products amplified from five out of the seven known genotypes of Lyssaviruses showed the stability of viral RNA viruses after fixation on the filter paper. Furthermore, the sensitivity of the hnRT-PCR following RNA fixation on the filter paper was equivalent to that of standard hnRT-PCR. In conclusion, the stability of viral RNA and the inactivation of infectivity make the FTA technology useful for the storage, transport, collection and subsequent molecular analysis of viral rabies RNA, facilitating epidemiological investigations in the field.
- Subjects :
- Animals
Base Sequence
Cell Line
Cloning, Molecular
Cricetinae
DNA Primers
Dogs
Evaluation Studies as Topic
Feasibility Studies
Filtration instrumentation
Filtration veterinary
Foxes
Lyssavirus genetics
Molecular Sequence Data
Nucleic Acid Amplification Techniques
RNA, Viral analysis
Reverse Transcriptase Polymerase Chain Reaction veterinary
Sensitivity and Specificity
Sequence Homology, Nucleic Acid
Specimen Handling instrumentation
Time Factors
Biotechnology methods
Paper
Rabies virus genetics
Rabies virus isolation & purification
Specimen Handling veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 0166-0934
- Volume :
- 140
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 17157394
- Full Text :
- https://doi.org/10.1016/j.jviromet.2006.11.011