Visible sensing of nucleic acid sequences using a genetically encodable unmodified mRNA probe.

Authors :: Narita A
Ogawa K
Sando S
Aoyama Y
Source :: Nucleic acids symposium series (2004) [Nucleic Acids Symp Ser (Oxf)] 2006 (50), pp. 283-4.
Publication Year :: 2006
Abstract: We previously reported a molecular beacon-mRNA (MB-mRNA) strategy for nucleic acid detection/sensing in a cell-free translation system using unmodified RNA as a probe. Here in this presentation, we report that a combination with RNase H activity, which induces an additional process of irreversible cleavage of MB-domain, achieves an improved sequence selectivity (one nucleotide selectivity) and an enhanced sensitivity. This improved system finally enabled visible sensing of target nucleic acid sequence at a single nucleotide resolution under isothermal conditions.

Subjects :: Base Sequence
Cell-Free System
Colorimetry
Fluorescence Resonance Energy Transfer
Genes, Reporter
Luciferases analysis
Luciferases genetics
Luminescent Agents analysis
Luminescent Measurements
Oligodeoxyribonucleotides analysis
Receptors, CCR5 genetics
beta-Galactosidase analysis
beta-Galactosidase genetics
Biosensing Techniques
Nucleic Acid Hybridization methods
Nucleic Acids analysis
Protein Biosynthesis
RNA Probes chemistry
Ribonuclease H

Full Text Access

Tools