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Neutrophils express the high affinity receptor for IgG (Fc gamma RI, CD64) after in vivo application of recombinant human granulocyte colony-stimulating factor.
- Source :
-
Blood [Blood] 1991 Aug 15; Vol. 78 (4), pp. 885-9. - Publication Year :
- 1991
-
Abstract
- Fc receptors are important effector molecules of neutrophilic granulocytes (polymorphonuclear neutrophils [PMN]), connecting phagocytic cells and the specific immune response. Neutrophils from healthy donors express the low-affinity receptors for IgG Fc gamma RII (CD32) and Fc gamma RIII (CD16), but not the high-affinity receptor Fc gamma RI (CD64). The latter has been found on neutrophils from patients with certain bacterial infections and can be induced in vitro after incubation with interferon-gamma. We show here that neutrophils strongly express Fc gamma RI after in vivo application of recombinant human granulocyte colony-stimulating factor (rhG-CSF). PMN from patients receiving rhG-CSF displayed higher cytotoxicity against Daudi lymphoma cells in vitro compared with control patients and with healthy donors. Fab fragments against Fc gamma RII (monoclonal antibody [MoAb] IV.3) inhibited neutrophil-mediated cytotoxicity of healthy donors but not of patients during rhG-CSF therapy. Therefore, expression of Fc receptors by PMN was investigated by flow cytometry and the mean fluorescence intensity (MFI) was compared. After staining with MoAb 32.2 against Fc gamma RL, the median MFI of neutrophils from G-CSF patients (median, 4.78; range, 2.40 to 8.50; n = 5) was significantly higher (P = .002 and P = .001, respectively) than the median MFI of patients not receiving G-CSF (median, 1.23; range, 1.01 to 1.58; n = 6) and the median MFI of healthy donors (median, 1.04; range, 0.67 to 1.12; n = 6). Fc gamma RI disappeared after the discontinuing of the G-CSF injections, but was reinduced during the next treatment cycle with rhG-CSF. The high expression of Fc gamma RI during rhG-CSF therapy correlated with enhanced cytotoxicity. In vitro incubation with rhG-CSF also enhances cytotoxicity, but only minor increments in Fc gamma RI expression were observed. Thus, during in vivo application of rhG-CSF neutrophils acquire an additional potent receptor for mediating tumor cell killing in vitro by induction of the high-affinity receptor for IgG (Fc gamma RI, CD64).
- Subjects :
- Antibodies, Monoclonal pharmacology
Antigens, Differentiation immunology
Carcinoma, Squamous Cell drug therapy
Carcinoma, Squamous Cell immunology
Cytotoxicity, Immunologic
Flow Cytometry
Granulocyte Colony-Stimulating Factor pharmacology
Humans
Immunoglobulin Fab Fragments pharmacology
Mouth Neoplasms drug therapy
Mouth Neoplasms immunology
Pharyngeal Neoplasms drug therapy
Pharyngeal Neoplasms immunology
Prospective Studies
Receptors, Fc immunology
Receptors, IgG
Recombinant Proteins pharmacology
Recombinant Proteins therapeutic use
Antigens, Differentiation metabolism
Granulocyte Colony-Stimulating Factor therapeutic use
Immunoglobulin G metabolism
Neutrophils immunology
Receptors, Fc metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 78
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 1714327