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Cloning, primary sequence, and chromosomal mapping of a human flavin-containing monooxygenase (FMO1).
- Source :
-
The Journal of biological chemistry [J Biol Chem] 1991 Jul 05; Vol. 266 (19), pp. 12379-85. - Publication Year :
- 1991
-
Abstract
- cDNA clones that code for a pig and human flavin-containing monooxygenase (FMO) have been isolated. The full-length sequence of the human cDNAs revealed that they encode a polypeptide of 532 amino acid residues containing putative FAD- and NADP-binding sites. The deduced amino acid sequence has 88 and 86% identity, respectively, with the pig and rabbit "hepatic" forms of FMO, but is only 58% similar to the rabbit "pulmonary" FMO, and thus represents the human ortholog of the "hepatic" form of FMO. However, as this FMO is present in low abundance in human adult liver, the general term "hepatic" for this form of the enzyme is misleading, and thus we propose the name FMO1 to describe this human FMO and its mammalian orthologs. Northern blot analysis demonstrated that human FMO1 mRNA is more abundant in fetal than in adult liver, indicating that in man the enzyme is subject to developmental regulation. Southern blot hybridization of human genomic DNA suggests that the protein is encoded by a single gene, which has been designated FMO1 and mapped to chromosome 1.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Blotting, Southern
Chromosomes, Human, Pair 1
Cloning, Molecular
DNA genetics
Humans
Hybrid Cells
Liver enzymology
Molecular Sequence Data
Polymerase Chain Reaction
RNA genetics
Rabbits
Sequence Homology, Nucleic Acid
Swine
Chromosome Mapping
Oxygenases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 266
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 1712018