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Regulation of Th2 cell development by Polycomb group gene bmi-1 through the stabilization of GATA3.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2006 Dec 01; Vol. 177 (11), pp. 7656-64. - Publication Year :
- 2006
-
Abstract
- The Polycomb group (PcG) gene products regulate the maintenance of the homeobox gene expression in Drosophila and vertebrates and also the cell cycle progression in thymocytes and Th2 cell differentiation in mature T cells. We herein studied the role of PcG gene bmi-1 product in Th1/Th2 cell differentiation and found that Bmi-1 facilitates Th2 cell differentiation in a Ring finger-dependent manner. Biochemical studies indicate that Bmi-1 interacts with GATA3 in T cells, which is dependent on the Ring finger of Bmi-1. The overexpression of Bmi-1 resulted in a decreased ubiquitination and an increased protein stability of GATA3. In bmi-1-deficient Th cells, the levels of Th2 cell differentiation decreased as the degradation and ubiquitination on GATA3 increased. Therefore, Bmi-1 plays a crucial role in the control of Th2 cell differentiation in a Ring finger-dependent manner by regulating GATA3 protein stability.
- Subjects :
- Animals
Cells, Cultured
DNA-Binding Proteins immunology
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
GATA3 Transcription Factor immunology
Immunoblotting
Immunoprecipitation
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Nuclear Proteins immunology
Polycomb Repressive Complex 1
Proto-Oncogene Proteins immunology
Repressor Proteins immunology
Reverse Transcriptase Polymerase Chain Reaction
Th1 Cells cytology
Cell Differentiation immunology
DNA-Binding Proteins metabolism
GATA3 Transcription Factor metabolism
Nuclear Proteins metabolism
Proto-Oncogene Proteins metabolism
Repressor Proteins metabolism
Th2 Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 177
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 17114435
- Full Text :
- https://doi.org/10.4049/jimmunol.177.11.7656