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Dicer1 expression in preimplantation mouse embryos: Involvement of Oct3/4 transcription at the blastocyst stage.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2007 Jan 05; Vol. 352 (1), pp. 231-6. Date of Electronic Publication: 2006 Nov 13. - Publication Year :
- 2007
-
Abstract
- Dicer1, an RNAse III enzyme, is a key factor for the production of microRNAs involved in post-transcriptional gene silencing. To elucidate the roles of Dicer1 and the microRNA pathway in early embryo development, we initially evaluated its gene expression in mouse oocytes and embryos in vitro. The transcript levels in GV stage oocytes steadily decreased up to the 2-cell embryo stage, and expression remained stable during morulae and blastocyst formation. DICER1 protein synthesis was additionally observed in mouse oocytes and early embryos. Silencing of mRNA expression by RNA interference (siRNA) did not inhibit development up to the blastocyst stage. Real-time RT-PCR experiments confirmed the decreased expression of selected transcription factors, including POU domain, class 5, transcription factor 1 (Pou5f1), SRY-box containing gene 2 (Sox2), and Nanog homeobox (Nanog). Moreover, POU5F1 protein expression was suppressed by Dicer1 siRNA. The results suggest that Dicer1 gene expression is associated with the levels of transcription factors, Pou5f1, Sox2, and Nanog which possibly regulate differentiation processes at the blastocyst stage.
- Subjects :
- Animals
Cell Differentiation
DEAD-box RNA Helicases genetics
Endoribonucleases genetics
Female
Mice
Oocytes cytology
Oocytes metabolism
RNA, Messenger genetics
RNA, Small Interfering genetics
Ribonuclease III
Blastocyst metabolism
DEAD-box RNA Helicases metabolism
Endoribonucleases metabolism
Gene Expression Regulation, Developmental
Octamer Transcription Factor-3 genetics
Transcription, Genetic genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 352
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 17113034
- Full Text :
- https://doi.org/10.1016/j.bbrc.2006.11.009